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首页> 外文期刊>Life sciences >DEMONSTRATION OF RIBONUCLEASE ACTIVITY IN THE PLANT RIBOSOME-INACTIVATING PROTEINS ALPHA- AND BETA-MOMORCHARINS
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DEMONSTRATION OF RIBONUCLEASE ACTIVITY IN THE PLANT RIBOSOME-INACTIVATING PROTEINS ALPHA- AND BETA-MOMORCHARINS

机译：植物核糖体失活蛋白α-和β-果糖中核糖核酸酶活性的证明

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Alpha- and beta-momorcharins, ribosome-inactivating proteins from Momordica charantia seeds, were utilized in this investigation. Ribonucleolytic cleavage was observed after naked rRNA was incubated with either momorcharin. Beta-momorcharin, and to a lesser extent alpha-momorcharin, also acted on tRNA to release acid-soluble UV-absorbing products. Such activity was optimal at pH around 5.5. Using polyhomoribonucleotides as substrate, it was found that the momorcharins preferentially acted on polyU, but exerted negligible effects on polyA, polyC and polyG. Chromatographic analysis of the reaction product indicated that mono and/or oligo-ribonucleotides, but not free base, were generated from polyU, suggesting that the enzymatic action involved ribonucleolytic cleavage. Similar to the results obtained with tRNA as substrate, beta-momorcharin was about 15-fold more active than alpha-momorcharin on polyU. [References: 21]
机译：这项研究利用了来自苦瓜种子的核糖体失活蛋白α-和β-mormorcharins。将裸露的rRNA与任一莫莫沙林孵育后，观察到核糖核酸裂解。 β-吗啡和较小程度的α-吗啡也作用于tRNA释放酸溶性紫外线吸收产物。这种活性在pH值约5.5时是最佳的。使用多核糖核苷酸作为底物，发现苦瓜素优先作用于polyU，但对polyA，polyC和polyG的作用可忽略不计。反应产物的色谱分析表明，由polyU产生了单核糖和/或寡核糖核苷酸，但没有游离碱基，这表明酶促作用涉及核糖核酸裂解。与以tRNA为底物获得的结果相似，在polyU上，β-吗啡的活性比α-吗啡的活性高约15倍。 [参考：21]

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