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首页> 外文期刊>Life sciences >EFFECT OF GROWTH HORMONE, INSULIN AND DEXAMETHASONE ON 11 BETA-HYDROXYSTEROID DEHYDROGENASE ACTIVITY ON A PRIMARY CULTURE OF RAT HEPATOCYTES
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EFFECT OF GROWTH HORMONE, INSULIN AND DEXAMETHASONE ON 11 BETA-HYDROXYSTEROID DEHYDROGENASE ACTIVITY ON A PRIMARY CULTURE OF RAT HEPATOCYTES

机译：生长激素，胰岛素和地塞米松对大鼠肝细胞原代培养物中11种β-羟基甾体脱氢酶活性的影响

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11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) is a microsomal enzyme present in the peripheral tissues of the rat, including the liver, and is mediated by a number of factors in animal in vivo studies. However, the effect of peptide hormones and glucocorticoids on the activity of 11 beta-HSD in isolated rat hepatocytes is not clear. To investigate these effects, we determined 11 beta-HSD activity in a primary culture of rat hepatocytes by adding various concentrations of growth hormone, insulin and dexamethasone (Dex). 11 beta-HSD activity increased significantly after treatment with Dex (10(-9)M-10(-6)M) for 48h. Dex (100nM) treated hepatocytes, incubated for 12h to 48h, resulted in a significant two - to four-fold rise in 11 beta-HSD activity compared to control (p<0.01), which was in contrast to GH (10(-9)M-10(-6)M) and insulin (10(-8)M-10(-5)M), which inhibited 11 beta-HSD activity (p<0.05). These results suggest that the 11 beta-HSD of rat hepatocytes is under multifactorial regulation; Dex stimulates and GH and insulin inhibit 11 beta-HSD activity in primary cultures of rat hepatocytes. [References: 36]
机译：11β-羟基类固醇脱氢酶（11β-HSD）是存在于大鼠外围组织（包括肝脏）中的微粒体酶，在动物体内研究中受多种因素介导。但是，尚不清楚肽激素和糖皮质激素对离体大鼠肝细胞中11β-HSD活性的影响。为了研究这些影响，我们通过添加各种浓度的生长激素，胰岛素和地塞米松（Dex），确定了大鼠肝细胞原代培养物中的11种β-HSD活性。用Dex（10（-9）M-10（-6）M）处理48小时后，11β-HSD活性显着增加。经Dex（100nM）处理的肝细胞孵育12h至48h，与对照组相比，11β-HSD活性显着提高了2-4倍（p <0.01），与GH（10（-9 ）M-10（-6）M）和胰岛素（10（-8）M-10（-5）M）抑制11β-HSD活性（p <0.05）。这些结果表明，大鼠肝细胞的11种β-HSD处于多因素调控之下。在大鼠肝细胞的原代培养物中，Dex刺激并且GH和胰岛素抑制11β-HSD活性。 [参考：36]

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