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首页> 外文期刊>Life sciences >Hyaluronic acid of high molecular weight inhibits proliferation and induces cell death in U937 macrophage cells
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Hyaluronic acid of high molecular weight inhibits proliferation and induces cell death in U937 macrophage cells

机译：高分子量透明质酸抑制U937巨噬细胞增殖并诱导细胞死亡

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摘要

Hyaluronic acid (HA), a major glycosaminoglycan component of the extracellular matrix, has regulatory influences on cells and cellular activities. To explore the effects of a high concentration (1 mg/mL) of high molecular weight HA (500-730 kD) on U937 macrophage growth dynamics, three factors that influence overall cellular growth, namely proliferation, apoptosis, and cell death, were examined. Cells were cultured with HA and were analyzed by flow cytometry every 24 hours during a 168-hour period for proliferation and the presence of apoptotic and dead cells. These analyses demonstrated that HA inhibits U937 macrophage proliferation in a time-dependent manner. Through the first 72 hours, cells exhibited slowed proliferation. However, no evidence of cell division arrest or reduced cell viability was observed. Thereafter, HA continued to diminish proliferation, but induced apoptosis. This data is consistent with regulatory influences secondary to HA binding to CD44 and/or RHAMM cell surface receptors, both of which were shown to be expressed on U937 macrophages. This study demonstrates that a high concentration of high molecular weight HA greatly inhibits macrophage population growth by the dual actions of impeding cell proliferation and inducing apoptosis. (C) 2004 Published by Elsevier Inc.
机译：透明质酸（HA）是细胞外基质的主要糖胺聚糖成分，对细胞和细胞活性具有调节作用。为了探索高浓度（1 mg / mL）高分子量HA（500-730 kD）对U937巨噬细胞生长动力学的影响，研究了影响总体细胞生长的三个因素，即增殖，凋亡和细胞死亡。 。用HA培养细胞，并在168小时内每24小时通过流式细胞术分析其增殖以及凋亡和死细胞的存在。这些分析表明HA以时间依赖性方式抑制U937巨噬细胞增殖。在最初的72小时内，细胞显示出缓慢的增殖。但是，没有观察到细胞分裂停滞或细胞活力降低的证据。此后，HA继续减少增殖，但诱导凋亡。该数据与HA结合CD44和/或RHAMM细胞表面受体继发的调节作用一致，这两者均显示在U937巨噬细胞上表达。这项研究表明高浓度的高分子量HA通过阻止细胞增殖和诱导细胞凋亡的双重作用极大地抑制了巨噬细胞的生长。 （C）2004由Elsevier Inc.出版

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