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首页> 外文期刊>Life sciences >Capsaicin induces the production of IL-6 in human upper respiratory epithelial cells.
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Capsaicin induces the production of IL-6 in human upper respiratory epithelial cells.

机译：辣椒素诱导人上呼吸道上皮细胞中IL-6的产生。

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摘要

Capsaicin, a type of alkaloid and the pungent component of chili peppers, is used as a therapeutic drug against allergic rhinitis and also as an index of bronchial hypersensitivity. Capsaicin receptor (TRPV1) expression has been identified in non-neuronal cells as well as neuronal cells. In our previous study, both TRPV1 protein and its gene expression on nasal epithelial cells were confirmed by immunohistochemistry and RT-PCR, respectively. In order to clarify whether or not TRPV1 acts as a functional receptor, we examined the effects of capsaicin on the production of IL-6 from primary cultured human airway epithelial cells at both protein and mRNA levels. Human nasal epithelial cells (HNECs) and normal human bronchial/tracheal epithelial cells (NHBE cells) were stimulated with increasing concentrations of capsaicin and/or pretreatment with capsazepine (TRPV1 antagonist) at 37 degrees C. The supernatant and total RNA were collected at 0, 4, 12, 24 and 48 h after treatment. IL-6 concentration and the IL-6 mRNA level were evaluated by ELISA and real-time PCR, respectively. Capsaicin (10 nM-10 muM) induced production of IL-6 from HNECs and NHBE cells and this effect was inhibited by pretreatment with capsazepine. Our findings suggest that topical application of capsaicin to the airway induces IL-6 production from respiratory epithelial cells via activation of TRPV1.
机译：辣椒素是一种生物碱，是辣椒的刺激性成分，被用作抗过敏性鼻炎的治疗药物，还用作支气管超敏反应的指标。辣椒素受体（TRPV1）的表达已在非神经元细胞和神经元细胞中得到鉴定。在我们先前的研究中，分别通过免疫组织化学和RT-PCR证实了TRPV1蛋白及其基因在鼻上皮细胞上的表达。为了阐明TRPV1是否充当功能受体，我们检查了辣椒素对蛋白质和mRNA水平上原代培养的人气道上皮细胞产生IL-6的影响。在37摄氏度下，通过增加浓度的辣椒素刺激人的鼻上皮细胞（HNEC）和正常的人支气管/气管上皮细胞（NHBE细胞）和/或用辣椒素（TRPV1拮抗剂）预处理。在0时收集上清液和总RNA治疗后第4、12、24和48小时。通过ELISA和实时PCR分别评估IL-6浓度和IL-6mRNA水平。辣椒素（10 nM-10 muM）诱导HNEC和NHBE细胞产生IL-6，而这种作用被辣椒碱预处理所抑制。我们的发现表明，辣椒素在气道上的局部应用可通过激活TRPV1诱导呼吸道上皮细胞产生IL-6。

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