• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Effects of 17beta-estradiol replacement on the apoptotic effects caused by ovariectomy in the rat hippocampus.
【24h】

Effects of 17beta-estradiol replacement on the apoptotic effects caused by ovariectomy in the rat hippocampus.

机译：17β-雌二醇替代对大鼠海马卵巢切除术引起的凋亡的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AIMS: The aim of the present study was to investigate the effects of different periods of ovariectomy and 17beta-estradiol replacement on apoptotic cell death and expression of members of the Bcl-2 family in the rat hippocampus. MAIN METHODS: Hippocampi were obtained from rats in proestrus, ovariectomized (15 days, 21 days and 36 days), ovariectomized for 15 days and then treated with 17beta-estradiol for 7 or 21 days, and rats ovariectomized and immediately treated with 17beta-estradiol for 21 days. The expression of Bcl-2 and Bax and the number of apoptotic cells were determined. KEY FINDINGS: Ovariectomy decreased Bcl-2 expression and increased Bax expression and the number of apoptotic cells. Replacement with 17beta-estradiol (21 days) throughout the post-ovariectomy period reduced the number of apoptotic cells to the control levels, and prevented the effects of ovariectomy on Bax expression, but only partially restored the Bcl-2 expression. After 15 days of ovariectomy, the replacement with 17beta-estradiol for 21 days, but not for 7 days, restored the Bcl-2 and Bax expression and the percentage of apoptotic cells to the levels found in the proestrus control. SIGNIFICANCE: The present results show that a physiological concentration of 17beta-estradiol may help maintain long-term neuronal viability by regulating the expression of members of the Bcl-2 family. Even after a period of hormonal deprivation, treatment with 17beta-estradiol is able to restore the expression of Bax and Bcl-2 to control levels, but the duration of the treatment is a key factor to obtain the desired effect. These data provide new understanding into the mechanisms contributing to the neuroprotective action of estrogen.
机译：目的：本研究的目的是研究不同时期的卵巢切除术和17β-雌二醇替代对大鼠海马凋亡细胞死亡和Bcl-2家族成员表达的影响。主要方法：从处于发情期的大鼠中取出海马，去卵巢（15天，21天和36天），去卵巢15天，然后用17β-雌二醇处理7或21天，将大鼠去卵巢，立即用17β-雌二醇治疗。持续21天。测定Bcl-2和Bax的表达以及凋亡细胞的数目。主要发现：卵巢切除术可降低Bcl-2表达，并增加Bax表达和凋亡细胞数量。在整个卵巢切除术后，用17β-雌二醇替代（21天）可将凋亡细胞的数量降至对照组水平，并防止卵巢切除对Bax表达的影响，但只能部分恢复Bcl-2表达。卵巢切除15天后，用17β-雌二醇替代21天而不是7天，将Bcl-2和Bax表达以及凋亡细胞的百分比恢复到了发情对照组中的水平。意义：目前的结果表明，生理浓度的17β-雌二醇可以通过调节Bcl-2家族成员的表达来帮助维持长期的神经元活力。即使经过一段时间的激素剥夺，用17β-雌二醇治疗也能够将Bax和Bcl-2的表达恢复到控制水平，但是治疗的持续时间是获得所需效果的关键因素。这些数据为促成雌激素神经保护作用的机制提供了新的认识。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号