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首页> 外文期刊>Life sciences >Dehydroepiandrosterone (DHEA) and DHEA-S interact with 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) to stimulate human osteoblastic cell differentiation.
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Dehydroepiandrosterone (DHEA) and DHEA-S interact with 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) to stimulate human osteoblastic cell differentiation.

机译：脱氢表雄酮（DHEA）和DHEA-S与1,25-二羟基维生素D3（1,25（OH）2D3）相互作用以刺激人类成骨细胞分化。

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摘要

DHEA, an adrenocortical steroid, and its sulfate derivative (DHEA-S), have been implicated in many biological functions, including the regulation of bone mass. In this study, we examined whether DHEA/DHEA-S are capable of directly affecting bone cell proliferation and differentiation, and compared this with the effects of, and interaction with, the established bone cell modulating steroid, 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). Two in vitro models of human osteoblastic cells were used, viz. MG63 osteosarcoma cell line and normal primary osteoblast-like cells (HOB). Our results show that DHEA and DHEA-S failed on their own to exert direct, independent significant effects on the growth and differentiation of human osteoblastic cells, but treating the cells in conjunction with 1,25(OH)2D3 resulted in enhancement of specific A1P activity. Moreover, 1,25(OH)2D3-induced osteocalcin production was potentiated by the adrenal steroids in both cell models. DHEA-S proved in general to be more potent than DHEA. In conclusion, this study shows that the effects of DHEA/DHEA-S on osteoblastic cell growth and differentiation are likely to be mediated via an effect on 1,25(OH)2D3-induced changes in bone cells, suggesting a distinctive role for these steroids in the regulation of bone metabolism.
机译：肾上腺皮质类固醇DHEA及其硫酸盐衍生物（DHEA-S）与许多生物学功能有关，包括调节骨量。在这项研究中，我们检查了DHEA / DHEA-S是否能够直接影响骨细胞的增殖和分化，并将其与已建立的骨细胞调节类固醇1,25-二羟基维生素D3（1 ，25（OH）2D3）。使用了人类成骨细胞的两种体外模型，即。 MG63骨肉瘤细胞系和正常的原代成骨样细胞（HOB）。我们的结果表明，DHEA和DHEA-S自身未能对人类成骨细胞的生长和分化产生直接，独立的显着影响，但与1,25（OH）2D3结合处理细胞会导致特异性A1P增强活动。此外，在两种细胞模型中，肾上腺类固醇均能增强1,25（OH）2D3诱导的骨钙素生成。一般而言，DHEA-S比DHEA更有效。总之，这项研究表明，DHEA / DHEA-S对成骨细胞生长和分化的影响很可能是通过对1,25（OH）2D3诱导的骨细胞变化的影响来介导的，表明了这些作用的独特作用类固醇在骨骼代谢中的调节。

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