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Effects on sleep and dopamine levels of microdialysis perfusion of cannabidiol into the lateral hypothalamus of rats.

机译：大麻对大鼠下丘脑外侧微灌流对睡眠和多巴胺水平的影响。

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摘要

AIMS: The major non-psychoactive component of Cannabis sativa, cannabidiol (CBD), displays a plethora of actions including wakefulness. In the present study, we addressed whether perfusing CBD via microdialysis into lateral hypothalamus (LH) during the lights-on period would modify the sleep-wake cycle of rats as well as the contents of dopamine (DA) collected from nucleus accumbens (AcbC). Additionally, we tested whether perfusion of CBD into LH would block the sleep rebound after a sleep deprivation period. MAIN METHODS: Electroencephalogram and electromyogram electrodes were implanted in rats as well as a guide-cannula aimed to LH or AcbC. CBD perfusion was carried out via cannulae placed into LH whereas contents of DA were collected from AcbC and analyzed using HPLC means. KEY FINDINGS: We found that microdialysis perfusion of CBD (30, 60, or 90 nM) into LH of rat enhances alertness and suppresses sleep. This effect was accompanied with an increase in DA extracellular levels collected from the AcbC. Furthermore, perfusion of CBD into LH after total sleep deprivation prevented the sleep rebound. SIGNIFICANCE: These findings enhance the investigation about the neurobiological properties of CBD on sleep modulation.
机译：目的：大麻的主要非精神活性成分，大麻二酚（CBD），显示出包括清醒在内的多种动作。在本研究中，我们研究了在开灯期间通过微透析将CBD灌注到下丘脑外侧（LH）是否会改变大鼠的睡眠觉醒周期以及从伏隔核（AcbC）收集的多巴胺（DA）的含量。此外，我们测试了在睡眠剥夺期后，将CBD注入LH是否会阻止睡眠反弹。主要方法：将脑电图和肌电图电极以及用于LH或AcbC的引导套管植入大鼠体内。通过放置在LH中的套管进行CBD灌注，而从AcbC收集DA的含量，并使用HPLC手段进行分析。主要发现：我们发现CBD（30、60或90 nM）向大鼠左心室的微透析灌注增强了机敏性并抑制了睡眠。该作用伴随着从AcbC收集的DA细胞外水平的增加。此外，在完全丧失睡眠后，将CBD灌注到LH中可防止睡眠反弹。意义：这些发现加强了关于CBD对睡眠调节的神经生物学特性的研究。

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