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首页> 外文期刊>Life sciences >STUDIES ON THE IMMUNOGLOBULIN-E SYSTEM OF THE COMMON MARMOSET IN COMPARISON WITH HUMAN DATA
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STUDIES ON THE IMMUNOGLOBULIN-E SYSTEM OF THE COMMON MARMOSET IN COMPARISON WITH HUMAN DATA

机译：普通MAR猴的免疫球蛋白E系统与人类数据的比较研究

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In the common marmoset (Callithrix jacchus jacchus) immunoglobulin E (IgE) serum levels and IgE synthesis of peripheral blood mononuclear cells (PBMC) in vitro were investigated in order to look for homologies to the human system. While IgE was not found in marmoset blood plasma with three commercial antihuman IgE-kits with monoclonal antibodies (mAbs), two other kits using polyclonal antibodies against human IgE revealed detectable IgE concentrations of up to 10 kU/liter in plasma samples of 19 out of 21 marmosets. In accord with human data, rhIL-4 showed biological functions under in vitro conditions in PBMC of the New World monkey. Proliferation, measured by H-3-thymidine incorporation, of isolated PBMC of marmosets could be induced by rhIL-4. FACScan analysis showed an enhanced expression of the low affinity IgE receptor CD23 (Fc epsilon RII) on CD20(+) B lymphocytes after incubation with rhIL-4. Furthermore, PBMC from marmosets could be stimulated by IL-4 alone or in combination with dexamethasone as well as with lipopolysaccharide (E. coli) to produce IgE in culture. The results indicate that Callithrix jacchus is using an IgE system that is rather similar to that of humans, although not completely identical. Antihuman mAbs and rhIL-4 can be used to investigate IgE regulation in vitro of marmoset PBMC. These data encourage the development of a primate animal model for studying possible modifications of the IgE system under pathological conditions to find new therapeutic strategies in atopic diseases. [References: 26]
机译：在普通mar猴（Callithrix jacchus jacchus）中，研究了免疫球蛋白E（IgE）的血清水平和体外外周血单个核细胞（PBMC）的IgE合成，以寻找与人系统的同源性。虽然三只商业化的带有单克隆抗体（mAbs）的抗人IgE试剂盒在mar猴血浆中均未发现IgE，但另外两种使用抗人IgE的多克隆抗体的试剂盒却在19种血浆样品中可检测到的IgE浓度高达10 kU /升。 21个mar猴。根据人类数据，rhIL-4在新大陆猴的PBMC中在体外条件下显示出生物学功能。通过r-3-胸腺嘧啶核苷掺入测量的measured猴的分离的PBMC的增殖可以由rhIL-4诱导。 FACScan分析显示与rhIL-4孵育后，CD20（+）B淋巴细胞上低亲和力IgE受体CD23（Fc epsilon RII）的表达增强。此外，来自mar猴的PBMC可以单独或与地塞米松以及脂多糖（大肠杆菌）结合使用IL-4刺激，以在培养物中产生IgE。结果表明，Callithrix jacchus使用的IgE系统与人的系统非常相似，尽管不完全相同。抗人mAbs和rhIL-4可用于研究mar猴PBMC的体外IgE调节。这些数据鼓励开发灵长类动物模型，以研究病理条件下IgE系统的可能修饰，以寻找特应性疾病的新治疗策略。 [参考：26]

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