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首页> 外文期刊>Cell biology international. >Alphavbeta6 is required in maintaining the intestinal epithelial barrier function
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Alphavbeta6 is required in maintaining the intestinal epithelial barrier function

机译：维持肠上皮屏障功能需要Alphavbeta6

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Epithelial barrier dysfunction is involved in a large number of diseases, but the pathogenesis is unclear. Integrin alphavbeta6 (avb6) in involved in the maintenance of the mucosal homeostasis. We have investigated the role of avb6 in maintaining the epithelial barrier function. Using T84 monolayers cultures, transepithelial electric resistance (TER) and permeability to ovalbumin (OVA) were measured as indicators of functioning. The antigenicity of OVA collected from the Transwell basal chambers was assessed using OVA-specific T cell proliferation. Knockdown of the avb6 genes increased the permeability of T84 monolayers to OVA, but did not affect TER. The deficiency of avb6-related hyperpermeability in T84 monolayers could be compensated by adding exogenous avb6 to the culture. The OVA samples collected from the basal chambers had strong antigenicity as it markedly induced the antigen specific T cell proliferation. Addition of recombinant avb6 blocked increases in permeability of T84 monolayers to OVA induced by tumor necrosis factor-α.
机译：上皮屏障功能障碍涉及多种疾病，但发病机理尚不清楚。整合素αvbeta6（avb6）参与维持粘膜稳态。我们已经研究了avb6在维持上皮屏障功能中的作用。使用T84单层培养物，经上皮电阻（TER）和对卵白蛋白的渗透性（OVA）被测量为功能指标。使用OVA特异性T细胞增殖评估从Transwell基底室收集的OVA的抗原性。敲除avb6基因可增加T84单层膜对OVA的通透性，但不影响TER。通过向培养物中添加外源avb6可以弥补T84单层中avb6相关的高通透性不足。从基底室收集的OVA样品具有很强的抗原性，因为它可以明显诱导抗原特异性T细胞增殖。重组avb6的添加阻止了肿瘤坏死因子-α诱导的T84单层对OVA的通透性增加。

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