Heat shock protein 32/heme oxygenase-1 protects mouse Sertoli cells from hyperthermia-induced apoptosis by CO activation of sGC signalling pathways

LiL.; LiC.-M.; WuJ.; HuangS.; WangG.-L.

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Heat shock protein 32/heme oxygenase-1 protects mouse Sertoli cells from hyperthermia-induced apoptosis by CO activation of sGC signalling pathways

机译：热休克蛋白32 /血红素加氧酶-1通过CO激活sGC信号通路保护小鼠支持细胞免受高热诱导的细胞凋亡

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Heat shock protein 32 (Hsp32)/heme oxygenase-1 (HO-1) may be a key enzyme for the protection of cells against stress. Its antiapoptotic effect has been attributed to its product, carbon monoxide (CO), in many types of cells. However, whether its antiapoptoticmechanismplays a role in Sertoli cells (SCs) is not yet clear. We hypothesise that Hsp32/HO-1 and CO generated fromit provide survival advantages in SCs by preventing apoptosis under heat exposure. After treatment of cultured SCs with hyperthermia and/or Hsp32/HO-1 activater hemin, apoptosiswas measured valuated by annexinV-FITC and caspase-3 activation. We have also analysed the Hsp32/HO-1-derived CO content of cultured media and cyclic guanosine monophosphate (cGMP) production by enzyme-linked immunosorbent assay (ELISA). Hyperthermia induced SCs apoptosis, while preincubation with hemin suppressed SC hyperthermia-induced apoptosis. Hyperthermia and/or hemin increase Hsp32/HO-1 gene expression and the production of CO, which, in turn, stimulates the generation of cGMP. The results suggest that Hsp32/HO-1 is a protective factor in heat-stressed SCs, and that CO generated from Hsp32/HO-1 is involved in the anti-apoptotic pathway.

机译：热激蛋白32（Hsp32）/血红素加氧酶-1（HO-1）可能是保护细胞抵抗压力的关键酶。在许多类型的细胞中，其抗凋亡作用归因于其产物一氧化碳（CO）。但是，其抗凋亡机制是否在支持细胞（SCs）中起作用尚不清楚。我们假设从中产生的Hsp32 / HO-1和CO通过防止热暴露下的细胞凋亡在SC中提供生存优势。用高温和/或Hsp32 / HO-1激活剂血红素处理培养的SC后，通过AnnexinV-FITC和caspase-3激活评估凋亡。我们还通过酶联免疫吸附测定（ELISA）分析了培养基中Hsp32 / HO-1衍生的CO含量和环状鸟苷单磷酸（cGMP）的产生。热疗诱导SCs凋亡，而与血红素预孵育抑制SC热疗诱导的细胞凋亡。体温过高和/或血红素升高Hsp32 / HO-1基因的表达和CO的产生，进而刺激cGMP的产生。结果表明，Hsp32 / HO-1是热应激SC中的保护因子，并且Hsp32 / HO-1产生的CO参与抗凋亡途径。

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《Cell biology international.》 |2014年第1期|共8页
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LiL.; LiC.-M.; WuJ.; HuangS.; WangG.-L.;
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正文语种 eng
中图分类细胞生物学;
关键词
Apoptosis; Carbon monoxide; Heat shock protein 32; Hyperthermia; Sertoli cells;

机译：凋亡;一氧化碳;热休克蛋白32;体温过高;Sertoli细胞;

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专利

1. Heat shock protein 32/heme oxygenase-1 protects mouse Sertoli cells from hyperthermia-induced apoptosis by CO activation of sGC signalling pathways [J] . LiL., LiC.-M., WuJ., Cell biology international. . 2014,第1期

机译：热休克蛋白32 /血红素加氧酶-1通过CO激活sGC信号通路保护小鼠支持细胞免受高热诱导的细胞凋亡
2. Targeting of heat-shock protein 32/heme oxygenase-1 in canine mastocytoma cells is associated with reduced growth and induction of apoptosis. [J] . Experimental Hematology: Official Publication of the International Society for Experimental Hematology . 2008,第11期

机译：犬肥大细胞瘤细胞中热休克蛋白32 /血红素加氧酶-1的靶向与减少的生长和诱导凋亡相关。
3. Heat shock protein 70 protects rat peritoneal mesothelial cells from advanced glycation end-products-induced epithelial-to-mesenchymal transition through mitogen-activated protein kinases/extracellular signal-regulated kinases and transforming growth factor-beta/Smad pathways [J] . Yang Jun, Zhu Tiechui, Liu Xiangdong, Molecular medicine reports . 2015,第6期

机译：热休克蛋白70通过促分裂原激活的蛋白激酶/细胞外信号调节激酶和转化生长因子-β/ Smad途径保护大鼠腹膜间皮细胞免于晚期糖基化终产物诱导的上皮向间充质转化
4. Degraded collagen fragments activate NF-kB and protect human smooth muscle cells against apoptosis through induction of inhibitor of apoptosis proteins (lAPs) [C] . K. von Wnuck Lipinski, N. Ferri, B. Levka Nordrhein-Westfa?lische Akademie der Wissenschaften . 2005

机译：降解的胶原片段激活NF-KB并通过诱导凋亡蛋白抑制剂（圈）保护人平滑肌细胞免受细胞凋亡
5. The Signal Recognition Particle (SRP)-dependent membrane trafficking pathway plays a role in the regulatory control of the heat-shock transcription factor, sigma32. [D] . Lim, Bentley. 2013

机译：信号识别颗粒（SRP）依赖性膜运输途径在热休克转录因子sigma32的调节控制中发挥作用。
6. Upregulation of heat shock protein 32 in Sertoli cells alleviates the impairments caused by heat shock-induced apoptosis in mouse testis [O] . Lian Li, Zhao-Yu Han, Cheng-Min Li, 2013

机译：Sertoli细胞中热激蛋白32的上调减轻了热激诱导的小鼠睾丸细胞凋亡引起的损伤
7. Dual Protective Effects of Flavonoids from Petasites japonicus against UVB-Induced Apoptosis Mediated via HSF-1 Activated Heat Shock Proteins and Nrf2-Activated Heme Oxygenase-1 Pathways [O] . Ki Mo Kim, A-Rang Im, Sanghyun Lee, 2017

机译：来自叶柄的双重保护作用japonicus 对UVB诱导的凋亡介导的通过 HSF-1活性热休克蛋白和NRF2-活化的血红素氧酶-1途径

Heat shock protein 32/heme oxygenase-1 protects mouse Sertoli cells from hyperthermia-induced apoptosis by CO activation of sGC signalling pathways

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