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首页> 外文期刊>Life sciences >Inhibitory effects on phospholipase A2 and antivenin activity of melanin extracted from Thea sinensis Linn
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Inhibitory effects on phospholipase A2 and antivenin activity of melanin extracted from Thea sinensis Linn

机译：茶提取物对磷脂酶A2的抑制作用和黑色素的抗维生素活性。

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Antivenin activity of melanin extracted from black tea (MEBT) was reported for the first time. The antagonistic effect of MEBT was evaluated for Agkistrodon contortrix laticinctus (broadbanded copperhead), Agkistrodon halys blomhoffii (Japanese mamushi), and Crotalus atrox (western diamondback rattlesnake) snake venoms administered i.p. to ICR mice. MEBT was injected i.p. immediately after the venom administration in dose of 3 mg per mouse in the same place of venom injection. MEBT demonstrated neutralization effect against all venoms tested. The greatest antivenin effect of MEBT was found against Japanese mamushi snake venom. In this case, half the mice died within 2.5 +/- 0.7 h after injection of 0.9 mg/kg of venom. An immediate injection of MEBT substantially reduced the toxic effect of venom and extended time at the 50% level of survival up to 52.3 +/- 2.3 h. The antivenin activity of MEBT is due to chelating of Ca++ and non-specific binding of phospholipase A2. The inhibitory effect of MEBT on phospholipase A2 assessed for different venoms was similar to that obtained with pure enzyme. Low toxicity of MEBT in combination with its antagonistic activity against different venoms may allow effective life-saving treatment against snakebites. Such application of MEBT is important when identification of the snake is impossible or if specific treatment is unavailable. (C) 2004 Elsevier Inc. All rights reserved. [References: 22]
机译：首次报道了从红茶中提取的黑色素（MEBT）的抗venin活性。对经腹膜内注射的Agkistrodon contortrix laticinctus（宽带铜头），Agkistrodon halys blomhoffii（日本mamushi）和Crotalus atrox（西方小菜响尾蛇）蛇毒进行了评估，评估了MEBT的拮抗作用。 ICR小鼠。经腹膜内注射MEBT。毒液给药后立即在同一位置注射每只小鼠3 mg剂量。 MEBT对所有测试的毒液均显示出中和作用。发现MEBT对日本ma猴蛇毒具有最大的抗蛇毒效果。在这种情况下，一半小鼠在注射0.9 mg / kg毒液后2.5 +/- 0.7小时内死亡。立即注射MEBT可以在50％的生存水平（最多52.3 +/- 2.3小时）内大大降低毒液的毒性作用并延长时间。 MEBT的抗静脉蛋白活性归因于Ca ++的螯合和磷脂酶A2的非特异性结合。对不同毒液评估的MEBT对磷脂酶A2的抑制作用类似于用纯酶获得的抑制作用。 MEBT的低毒性加上其对不同毒液的拮抗活性，可以有效地挽救生命，预防蛇咬伤。当无法识别蛇或没有特定治疗方法时，MEBT的这种应用很重要。 （C）2004 Elsevier Inc.保留所有权利。 [参考：22]

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