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首页> 外文期刊>Life sciences >Testosterone effect on immature prostate gland development associated with suppression of transforming growth factor-beta.
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Testosterone effect on immature prostate gland development associated with suppression of transforming growth factor-beta.

机译：睾丸激素对未成熟前列腺发育的抑制作用与转化生长因子-β的抑制有关。

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The aim of this study was to evaluate the effect of testosterone treatment on the pattern of prostate cell proliferation and differentiation and their correlation with the expression of transforming growth factor-beta (TGF-beta). Prostate gland development was compared in intact immature dogs with one-month testosterone-treated immature dogs. Testosterone treatment resulted in a tenfold increase in prostate gland weight compared to untreated dogs, with a typical organization of the gland into a structure similar to that observed in mature dogs. The narrow acini which contain flat basal cells in immature glands were transformed into tubuloacinar structures containing columnar secretory cells and basal cells. The stromal compartments showed an increase in the muscular component as evidenced by the high reactivity to alpha-actin with no remarkable changes in the vimentin expression. In addition, testosterone treatment induced a significant reduction in the proliferation capacity of stromal cells but with no noticeable changes in the proliferation pattern of epithelial cells. These changes in the prostate are associated with a twofold decrease in TGF-beta mRNA expression as assessed by Real-Time PCR. However, the immunolocalization of TGF-beta was shifted slightly from the epithelial cells in untreated animals to the stromal cells of treated animals. Based on these results it appears that testosterone acts to coordinate prostatic cell proliferation and differentiation and direct their organization into a structure resembling that of the mature gland. The testosterone regulation of the prostate gland appears to involve the regulation of TGF-beta gene expression.
机译：这项研究的目的是评估睾丸激素治疗对前列腺细胞增殖和分化模式的影响及其与转化生长因子-β（TGF-β）表达的相关性。比较了完整未成熟狗和经睾丸激素治疗一个月的未成熟狗的前列腺发育。与未经治疗的犬相比，睾丸激素治疗导致前列腺腺体重量增加了十倍，腺体的典型组织结构类似于在成年犬中观察到的结构。在未成熟腺体中含有扁平基底细胞的狭窄腺泡被转化为含有柱状分泌细胞和基底细胞的微管腺结构。对α-肌动蛋白具有高反应性，而波形蛋白表达没有明显变化，证明了间质隔室的肌肉成分增加。另外，睾丸激素治疗引起基质细胞的增殖能力显着降低，但是上皮细胞的增殖模式没有明显变化。如通过实时PCR所评估的，前列腺中的这些变化与TGF-βmRNA表达的两倍降低有关。但是，TGF-β的免疫定位从未治疗动物的上皮细胞略微转移到了治疗动物的基质细胞。根据这些结果，睾丸激素似乎可以协调前列腺细胞的增殖和分化，并指导其组织形成类似于成熟腺体的结构。前列腺的睾丸激素调节似乎涉及TGF-β基因表达的调节。

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