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首页> 外文期刊>Life sciences >Expression of T-cell immunogrlobulin- and mucin-domain-containing molecule-1 (TIM-1) is increased in a mouse model of asthma and relationship to GATA-3
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Expression of T-cell immunogrlobulin- and mucin-domain-containing molecule-1 (TIM-1) is increased in a mouse model of asthma and relationship to GATA-3

机译：哮喘小鼠模型中T细胞免疫球蛋白和粘蛋白结构域的分子1（TIM-1）的表达增加并与GATA-3相关

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It has been suggested that T-cell immunoglobulin-and mucin-domain-containing molecule-1 (TIM-1) plays an important role in the development of allergic asthma, though its molecular mechanism remains unclear. Our aim was to examine the expression of TIM-1 and Th2-associated transcription factor GATA-3 in asthmatic mice, and to evaluate the correlation of TIM-1 and GATA-3 in the pathogenesis of allergic asthma. We examined TIM-1 expression in lung tissue and peripheral blood mononuclear cells (PBMCs) of asthmatic mice by real-time PCR and flow cytometry, respectively; we also investigated TIM-1 and GATA-3 expression in the spleen tissue of asthmatic mice by western blot. These results demonstrate that TIM-1 was significantly increased in pulmonary tissues and PBMCs in asthmatic mice after ovalbumin (OVA) challenge (P<0.05), and that the production of TIM-1 as well as GATA-3 was upregulated in the spleen of asthmatic mice. The production of TIM-1 correlated significantly with the production of GATA-3 in the spleen of asthmatic mice (r=0.753, P<0.05). The results of this study provide the first evidence that increased expression of TIM-1 in asthmatic mice is associated with the Th2-associated transcription factor GATA-3. The findings suggest a possible mechanism for how HAV infection and TIM-1 upregulation influence the development of allergic asthma. (C) 2008 Elsevier Inc. All rights reserved.
机译：有人提出，尽管尚不清楚其分子机制，但T细胞免疫球蛋白和粘蛋白结构域分子-1（TIM-1）在过敏性哮喘的发展中起着重要作用。我们的目的是检查哮喘小鼠中TIM-1和Th2相关转录因子GATA-3的表达，并评估TIM-1和GATA-3在过敏性哮喘发病中的相关性。我们分别通过实时荧光定量PCR和流式细胞术检测了哮喘小鼠肺组织和外周血单个核细胞（PBMC）中的TIM-1表达;我们还通过免疫印迹研究了哮喘小鼠脾脏组织中TIM-1和GATA-3的表达。这些结果表明，在卵清蛋白（OVA）攻击后，哮喘小鼠的肺组织和PBMC中TIM-1显着增加（P <0.05），并且TIM-1以及GATA-3的产生在脾脏中被上调哮喘小鼠。哮喘小鼠脾脏中TIM-1的产生与GATA-3的产生显着相关（r = 0.753，P <0.05）。这项研究的结果提供了第一个证据，即哮喘小鼠中TIM-1表达的增加与Th2相关转录因子GATA-3有关。这些发现提示了HAV感染和TIM-1上调如何影响过敏性哮喘的发展的可能机制。 （C）2008 Elsevier Inc.保留所有权利。

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