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首页> 外文期刊>Life sciences >Molecular basis of voltage-dependent delayed rectifier K+ channels in smooth muscle cells from rat tail artery.
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Molecular basis of voltage-dependent delayed rectifier K+ channels in smooth muscle cells from rat tail artery.

机译：大鼠尾动脉平滑肌细胞中依赖电压的延迟整流K +通道的分子基础。

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摘要

The molecular basis of voltage-dependent K+ (Kv) current in smooth muscle cells (SMCs) from rat tail artery was investigated by screening transcriptional expression of 15 Kv channel alpha-subunits and 3 Kv beta-subunits using RT-PCR technique. Among Kv genes that encode delayed rectifier Kv currents, mRNAs of Kv1.2, Kv1.3, Kv1.5, Kv2.1, Kv2.2, and Kv3.2 were expressed, but those of Kv1.1, Kv1.6, and Kv3.1 were not detected. The transient outward Kv current-encoding genes Kv1.4, Kv3.3, Kv3.4, Kv4.1- Kv4.3 as well as Kvbeta1, Kvbeta2, and Kvbeta3 were also expressed at mRNA level. Western blot study demonstrated the presence of Kv1.2, Kv1.3, Kv1.5, and Kv2.1, but not Kv3.2 proteins, in tail artery tissue. Immunocytochemistry study confirmed the presence of Kv1.2, Kv1.3, Kv1.5, and Kv2.1 channel proteins in primary cultured single SMCs. Our results represent the first systematic characterization of Kv gene expression in rat tail artery SMCs.
机译：通过使用RT-PCR技术筛选15 Kv通道α亚基和3 Kvβ亚基的转录表达，研究了大鼠尾动脉平滑肌细胞（SMC）中电压依赖性K +（Kv）电流的分子基础。在编码延迟整流Kv电流的Kv基因中，表达了Kv1.2，Kv1.3，Kv1.5，Kv2.1，Kv2.2和Kv3.2的mRNA，但Kv1.1，Kv1.6，和Kv3.1未检测到。瞬时向外Kv电流编码基因Kv1.4，Kv3.3，Kv3.4，Kv4.1-Kv4.3以及Kvbeta1，Kvbeta2和Kvbeta3也在mRNA水平表达。 Western blot研究表明尾动脉组织中存在Kv1.2，Kv1.3，Kv1.5和Kv2.1，但不存在Kv3.2蛋白。免疫细胞化学研究证实，在原代培养的单个SMC中存在Kv1.2，Kv1.3，Kv1.5和Kv2.1通道蛋白。我们的结果代表了大鼠尾动脉SMC中Kv基因表达的第一个系统表征。

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