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首页> 外文期刊>Cell biology international. >Cell death in Tetrahymena thermophila: new observations on culture conditions.
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Cell death in Tetrahymena thermophila: new observations on culture conditions.

机译：嗜热四膜虫的细胞死亡：培养条件的新观察。

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摘要

We previously suggested that the cell fate of the protozoan ciliate, Tetrahymena thermophila, effectively relates to a quorum-sensing mechanism where cell-released factors support cell survival and proliferation. The cells have to be present above a critical initial density in a chemically defined nutrient medium in order to release a sufficient level of these factors to allow a new colony to flourish. At a relatively high rate of metabolism and/or macromolecular synthesis and below this critical density, cells began to die abruptly within 30 min of inoculation, and this death took the form of an explosive disintegration lasting less than 50 milliseconds. The cells died at any location in the culture, and the frequency of cell death was always lower in well-filled vials than those with medium/air interface. Cell death was inhibited by the addition of Actinomycin D or through modifications of the culture conditions either by reducing the oxygen tension or by decreasing the temperature of the growth medium. In addition, plastic caps in well-filled vials release substances, which promote cell survival. The fate of low-density cultures is related to certain 'physical' conditions, in addition to the availability of oxygen within closed culture systems. Copyright 2001 Academic Press.
机译：我们以前曾提出，原生动物纤毛虫嗜热四膜虫的细胞命运有效地涉及群体感应机制，其中细胞释放的因子支持细胞存活和增殖。为了释放足够水平的这些因子以使新菌落蓬勃发展，细胞必须存在于化学定义的营养培养基中高于临界初始密度的位置。在相对较高的新陈代谢率和/或大分子合成速率下，并且低于此临界密度，细胞在接种后30分钟内开始突然死亡，并且这种死亡以爆炸性崩解的形式持续了不到50毫秒。细胞在培养物中的任何位置都死亡，并且在充满液体的小瓶中，细胞死亡的频率始终低于具有中等/空气界面的细胞。通过加入放线菌素D或通过降低氧张力或降低生长培养基的温度来改变培养条件来抑制细胞死亡。此外，装满小瓶的塑料盖会释放物质，从而促进细胞存活。低密度培养物的命运与某些“物理”条件有关，除了封闭培养系统中氧气的可用性外。版权所有2001，学术出版社。

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