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首页> 外文期刊>Cell biology international. >cGMP-regulated store-operated calcium entry in human hepatoma cells.
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cGMP-regulated store-operated calcium entry in human hepatoma cells.

机译:cGMP调节人肝癌细胞中的钙池操纵钙离子进入。

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摘要

This study aimed to investigate cGMP-regulated store-operated Ca(2+)entry in human 7721 hepatoma cells. [Ca(2+)](i)was measured using Fura2/AM. After incubation of the cells with 4 microm thapsigargin, Ca(2+)entry was evoked by application of 1 mMm Ca(2+)to extracellular solution and was blocked by 3 m m Ni(2+), indicating the presence of store-operated Ca(2+)entry in human 7721 hepatoma cell line. Application of 8-Br-cGMP reduced the [Ca(2+)](i)in hepatoma 7721 cells by 80%. These data demonstrated for the first time that store-operated Ca(2+)entry pathway is present in human hepatoma cells, which is regulated by cGMP.
机译:这项研究旨在调查人类7721肝癌细胞中cGMP调节的存储操作的Ca(2+)条目。 [Ca(2 +)](i)使用Fura2 / AM测量。在将细胞与4微米毒胡萝卜素孵育后,通过向细胞外溶液中施加1 mMm Ca(2+)诱发Ca(2+)进入,并被3 mm Ni(2+)阻断,表明存在由存储操作的Ca(2+)进入人类7721肝癌细胞系。 8-Br-cGMP的应用减少了肝癌7721细胞中的[Ca(2 +)](i)80%。这些数据首次证明在人类肝癌细胞中存在由cGMP调节的存储操作的Ca(2+)进入途径。

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