• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Intercellular adhesion molecule-1 and vascular endothelial growth factor expression kinetics in macrophage-derived foam cells.
【24h】

Intercellular adhesion molecule-1 and vascular endothelial growth factor expression kinetics in macrophage-derived foam cells.

机译：巨噬细胞源性泡沫细胞中细胞间粘附分子-1和血管内皮生长因子的表达动力学。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Macrophage-derived foam cells seem to play an important role during inflammatory response of atherosclerosis, in which the overexpression of intercellular adhesion molecule-1 (ICAM-1) and vascular endothelial growth factor (VEGF) are associated with the early and later pathological changes in foam cell formation. In this study, we investigated the expression kinetics of ICAM-1 and VEGF in macrophage-derived foam cells. The foam cell model was established through incubating the human monocyte line (U937 cells) with oxidized-low density lipoprotein (ox-LDL). Up-regulated expressions of ICAM-1 and VEGF were analyzed in protein and mRNA levels in U937 foam cells by flow cytometry, ELISA, and Northern blot. Kinetic studies showed the deferent kinds of expression curves in dose response and time course. The expression dose-kinetics demonstrated that the ICAM-1 showed the peak expression induced by ox-LDL 50 mg/L, while VEGF levels increased in a dose-dependent manner with the maximum level induced by ox-LDL 200 mg/L. Time-kinetic studies revealed that the ICAM-1 levels showed the peak expression in 12 h while VEGF expression increased in a time-dependent manner with the maximum level in 48 h. These results proved that both ICAM-1 and VEGF expressions were enhanced in the macrophage-derived foam cells, but ICAM-1 expression increased earlier than the up-regulation of VEGF; low dose of ox-LDL mainly up regulated ICAM-1 expression, while high dose mainly increased the VEGF expression.
机译：巨噬细胞源性泡沫细胞似乎在动脉粥样硬化的炎症反应中起重要作用，其中细胞间粘附分子-1（ICAM-1）和血管内皮生长因子（VEGF）的过表达与早期和晚期病理改变有关。泡沫孔形成。在这项研究中，我们调查了ICAM-1和VEGF在巨噬细胞源性泡沫细胞中的表达动力学。通过将人类单核细胞系（U937细胞）与氧化的低密度脂蛋白（ox-LDL）孵育来建立泡沫细胞模型。通过流式细胞仪，ELISA和Northern blot分析U937泡沫细胞中ICAM-1和VEGF的表达上调。动力学研究表明剂量反应和时间过程中不同的表达曲线。表达剂量动力学表明，ICAM-1显示出ox-LDL 50 mg / L诱导的峰值表达，而VEGF水平以剂量依赖性方式增加，而ox-LDL 200 mg / L诱导的最大水平。时间动力学研究表明，ICAM-1水平在12 h达到峰值，而VEGF表达以时间依赖性方式增加，在48 h达到最高水平。这些结果证明，巨噬细胞源性泡沫细胞中ICAM-1和VEGF的表达均增强，但ICAM-1的表达比VEGF的上调更早。低剂量的ox-LDL主要上调ICAM-1表达，而高剂量的主要上调VEGF表达。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号