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首页> 外文期刊>Life sciences >Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice.
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Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice.

机译：鼻内螨过敏原在NC / Nga小鼠中诱发类似哮喘的过敏反应。

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Airway responses induced by intranasal administration of mite allergen without adjuvant were studied in NC/Nga mice. A crude extract of Dermatophagoides farinae (Df) was administered for 5 consecutive days and a single intranasal challenge booster dose was given 1 week after the last sensitization. 24 h after the single challenge, the airway hyperresponsiveness (AHR) was measured and the bronchoalveolar lavage fluid (BALF) was analyzed for numbers of eosinophils and neutrophils, and both cytokine and chemokine levels. There were marked increases in number of eosinophils in the BALF, AHR, Th2 cytokines (IL-5 and IL-13), and chemokine (eotaxin-1 and eotaxin-2) levels in the BALF following Df exposure. C57BL/6N, A/J, BALB/c, and CBA/JN mouse strains were also exposed to Df crude extract, but all of the measured responses were strongest in NC/Nga mice. Furthermore, Df-exposed NC/Nga mice showed the goblet cell hyperplasia, pulmonary eosinophilic inflammation, and increases in both total serum IgE and Df-specific IgG1. After intranasal exposure of NC/Nga mice to crude extract of Dermatophagoides pteronyssinus, the BALF eosinophilia and AHR were similar to responses induced by Df. None of the study parameters were increased in response to intranasal exposure to ovalbumin. These data demonstrated that NC/Nga mice developed allergic asthma-like responses after intranasal exposure to mite allergens.
机译：在NC / Nga小鼠中研究了通过鼻内施用无佐剂的螨过敏原诱导的气道反应。连续5天服用法氏皮肤癣菌（Dermatophagoides farinae，Df）的粗提物，并在最后一次致敏后1周给予一次鼻内激发加强剂量。单次攻击后24小时，测量气道高反应性（AHR），并分析支气管肺泡灌洗液（BALF）的嗜酸性粒细胞和中性粒细胞数量，以及细胞因子和趋化因子水平。暴露于Df后，BALF中的嗜酸性粒细胞数量明显增加，BALF中的嗜酸性粒细胞数量（IL-5和IL-13）以及趋化因子（eotaxin-1和eotaxin-2）的水平增加。 C57BL / 6N，A / J，BALB / c和CBA / JN小鼠品系也暴露于Df粗提物中，但所有测得的响应在NC / Nga小鼠中最强。此外，暴露于Df的NC / Nga小鼠显示出杯状细胞增生，肺嗜酸性粒细胞炎症，并且总血清IgE和Df特异性IgG1均增加。在鼻内暴露NC / Nga小鼠翼状ter肉的粗提物中后，BALF嗜酸性粒细胞增多和AHR与Df诱导的反应相似。鼻内暴露于卵清蛋白后，没有增加任何研究参数。这些数据表明，NC / Nga小鼠经鼻内暴露于螨过敏原后会出现类似哮喘的过敏反应。

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