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首页> 外文期刊>Cell and Tissue Research >IL-10 secreting CD21? B cells are present in jejunal Peyer's patches of sheep during fetal development.
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IL-10 secreting CD21? B cells are present in jejunal Peyer's patches of sheep during fetal development.

机译：IL-10分泌CD21吗？胎儿发育过程中，绵羊空肠Peyer斑中存在B细胞。

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摘要

We recently reported a novel interleukin-10 (IL-10)-secreting CD21(+) B cell population in jejunal Peyer's patches (JPP) of sheep with a regulatory function (Bregs) suppressing Toll-like receptor 9 (TLR9)-induced cytokine responses. However, little is known about the development of these cells. Therefore, we investigate their existence in JPP cells from fetal and newborn lambs. CD21(+) B cells were purified from JPP cells by magnetic cell sorting and subsequently stimulated with the TLR9 agonist, CpG ODN (CpG oligodeoxynucleotide). Lymphocyte proliferative responses, cytokine production (IL-10, IL-12 and interferon-γ [INF-γ]) and antibody secretion were assayed. We found that fetal and neonatal CD21(+) B cells spontaneously secreted high levels of IL-10 regardless of CpG stimulation but that these cells did not produce any IL-12 or INF-γ upon stimulation with CpG. The observed responses are consistent with those previously reported for Bregs characterized in JPP of older lambs. Surprisingly, unlike in older lambs, fetal and neonatal JPP CD21(+) B cells proliferated in response to CpG stimulation. Our investigations of fetal and neonatal lambs provide evidence for the development of IL-10-secreting CD21(+) B cells in PPs prior to antigen exposure.
机译：我们最近报道了绵羊的空肠Peyer斑（JPP）具有调节功能（Bregs）抑制Toll样受体9（TLR9）诱导的细胞因子的分泌白介素10（IL-10）的新型CD细胞群体。回应。但是，关于这些细胞的发育知之甚少。因此，我们调查了它们在胎儿和新生羔羊的JPP细胞中的存在。通过磁性细胞分选从JPP细胞中纯化CD21（+）B细胞，然后用TLR9激动剂CpG ODN（CpG寡脱氧核苷酸）刺激。测定淋巴细胞增殖反应，细胞因子产生（IL-10，IL-12和干扰素-γ[INF-γ]）和抗体分泌。我们发现，无论CpG刺激如何，胎儿和新生儿CD21（+）B细胞都会自发分泌高水平的IL-10，但这些细胞在受CpG刺激后不会产生任何IL-12或INF-γ。观察到的反应与先前报道的以老羔羊的JPP为特征的Bregs的反应一致。出人意料的是，与年长的羔羊不同，胎儿和新生儿的JPP CD21（+）B细胞响应CpG刺激而增殖。我们对胎儿和新生羔羊的研究为抗原暴露前PPs中IL-10分泌CD21（+）B细胞的发育提供了证据。

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