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首页> 外文期刊>Life sciences >Placental/umbilical cord blood-derived mesenchymal stem cell-like stromal cells support hematopoietic recovery of X-irradiated human CD34+ cells.
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Placental/umbilical cord blood-derived mesenchymal stem cell-like stromal cells support hematopoietic recovery of X-irradiated human CD34+ cells.

机译：胎盘/脐带血来源的间充质干细胞样基质细胞支持X射线照射的人CD34 +细胞的造血恢复。

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摘要

AIMS: The potential of human mesenchymal stem cell-like stroma prepared from placental/umbilical cord blood for hematopoietic regeneration by X-irradiated hematopoietic stem cells is herein assessed. MAIN METHODS: Placental/umbilical cord blood-derived mesenchymal stem cell-like stromal cells were applied to a regenerative ex vivo expansion of X-irradiated human CD34(+) cells in a serum-free liquid culture supplemented with a combination of interleukine-3 plus stem cell factor plus thrombopoietin. KEY FINDINGS: The total number of cells and of lineage-committed myeloid hematopoietic progenitor cells generated in the co-culture of both non-irradiated and X-irradiated cells with stromal cells was significantly higher than those in the stroma-free culture. In addition, the number of CD34(+) cells and CD34(+)/CD38(-) cells, immature hematopoietic stem/progenitor cells also increased more than the stroma-free culture. The stromal cells produced various types of cytokines, although there was little difference between the co-cultures of non-irradiated and X-irradiated cells with stromal cells. Furthermore, when X-irradiated cells came in contact with stromal cells for 16 h before cytokine stimulation, a similar degree of hematopoiesis was observed, thus suggesting the critical role of cell-to-cell interaction. SIGNIFICANCE: The present results showed the potential efficacy of human mesenchymal stem cell-like stroma for hematopoietic regeneration from irradiated hematopoietic stem/progenitor cells.
机译：目的：本文评估了由胎盘/脐带血制备的人间充质干细胞样基质通过X射线照射的造血干细胞进行造血再生的潜力。主要方法：胎盘/脐血间充质干细胞样基质细胞应用于无血清液体培养物中补充X射线照射的人CD34（+）细胞的体外离体扩增，补充了白细胞介素3再加上干细胞因子再加上血小板生成素。主要发现：在未辐照和X辐照的细胞与基质细胞的共培养中，细胞总数和沿谱定型的骨髓造血祖细胞的总数均显着高于无基质培养的细胞总数。此外，CD34（+）细胞和CD34（+）/ CD38（-）细胞，未成熟的造血干/祖细胞的数量也比无基质培养物增加更多。基质细胞产生各种类型的细胞因子，尽管未辐照和X辐照的细胞与基质细胞的共培养之间几乎没有差异。此外，当X射线照射的细胞在细胞因子刺激之前与基质细胞接触16 h时，观察到相似程度的造血作用，因此表明细胞间相互作用的关键作用。意义：本研究结果显示了人间充质干细胞样基质对于辐射的造血干/祖细胞再生造血的潜在功效。

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