...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell and Tissue Research >Mucosal layers and related nerve fibres in non-chagasic and chagasic human colon--a quantitative immunohistochemical study.
【24h】

Mucosal layers and related nerve fibres in non-chagasic and chagasic human colon--a quantitative immunohistochemical study.

机译：非Chachaic和Chachaic人结肠中的粘膜层和相关神经纤维-定量免疫组织化学研究。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Chagasic megacolon is accompanied by extensive myenteric and, simultaneously, moderate submucosal neuron loss. Here, we examined changes of the innervation pattern of the lamina propria (LP) and muscularis mucosae (MM). Two alternating sets of cryosections were taken from seven non-chagasic colonic and seven chagasic megacolonic specimens (the latter included both the dilated megacolonic and the non-dilated transitional oral and anal zones) and were immunohistochemically triple-stained for smooth-muscle actin (SMA), synaptophysin (SYN) and glial acid protein S100 and, alternatively, for SMA, vasoactive intestinal peptide (VIP) and somatostatin (SOM). Subsequent image analysis and statistical evaluation of nervous tissue profile areas revealed that, in LP, the most extreme differences (i.e. increase in thickness or decrease in nerve, glia and muscle tissue profile area, respectively) compared with control values occurred in the dilated megacolonic zone itself. In contrast, the most extreme differences in the MM were in the anal-to-megacolonic zone (except the profile area of muscle tissue, which was lowest in the megacolonic zone). This parallels our previous results in the external muscle coat. A partial and selective survival of VIP-immunoreactive in contrast to SOM-immunoreactive nerve fibres was observed in both mucosal layers investigated. Thus, VIPergic nerve elements might be crucial for the maintenance of the mucosal barrier. The differential changes of neural tissue parameters in LP and MM might reflect a multifactorial rather than a pure neurogenic development of megacolon in chronic Chagas' disease.
机译：恰加斯加巨结肠伴有广泛的肠系膜，同时伴有中度粘膜下神经元丢失。在这里，我们检查了固有层（LP）和粘膜肌层（MM）的神经支配模式的变化。从七个非chachaic结肠结肠和七个chagasic megacolonic标本（后者包括扩张的megacolonic和未扩张的过渡口腔和肛门区）中抽取了两组交替的冷冻切片，并对其进行了免疫组织化学三重染色以检测平滑肌肌动蛋白（SMA） ），突触素（SYN）和神经胶质酸蛋白S100，或者对于SMA，血管活性肠肽（VIP）和生长抑素（SOM）。随后的图像分析和神经组织轮廓区域的统计评估表明，在LP中，与对照值相比，最大的差异（即厚度增加或神经，神经胶质和肌肉组织轮廓区域的减少分别）发生在扩张的大结肠区域本身。相反，MM的最大差异是在肛门至大结肠区域（肌肉组织的轮廓区域除外，该区域在大结肠区域最低）。这与我们先前在外层肌肉外套中的结果相似。与SOM免疫反应性神经纤维相反，在所研究的两个粘膜层均观察到VIP免疫反应性的部分和选择性存活。因此，VIPergic神经元可能对维持粘膜屏障至关重要。 LP和MM中神经组织参数的差异性变化可能反映了慢性Chagas病中巨结肠的多因素而非纯神经源性发展。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号