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首页> 外文期刊>Life sciences >Expression of matrix metalloproteinases in ovarian endometriomas: immunohistochemical study and enzyme immunoassay.
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Expression of matrix metalloproteinases in ovarian endometriomas: immunohistochemical study and enzyme immunoassay.

机译：基质金属蛋白酶在卵巢子宫内膜瘤中的表达：免疫组织化学研究和酶免疫法。

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Like carcinoma, endometriosis has the unique characteristics, of invasion and metastasis, though pathologically, it is a benign tumor. However, the mechanism of destruction of the surrounding tissue in endometriosis is still unclear. In this study, the expression and localization of matrix metalloproteinases (MMP)-1, -2, -3, -7, -9 and tissue inhibitors of metalloproteinases-1 (TIMP-1) were evaluated by immunohistochemistry for 20 cases and the amounts of MMP-1, TIMP-1 and MMP-1/TIMP-1 complex in the fluid of endometrioma, were analyzed by ELISA and western blotting for 20 cases, which were analyzed by immunohistochemical study. MMP-1, -2 and -9 were detected strongly in both stromal and epithelial cells and MMP-7 in the epithelial cells in the menstrual period. MMP-3 was mainly expressed in macrophage containing hemosiderin but the change of expression was not clear. TIMP-1 was intensively detected in both stromal and epithelial cells in the menstrual period but the expression decreased in other stages of the menstrual cycle. ELISA for MMP-1 also showed results similar to immunohistochemistry, suggesting that it was released to the cyst in the menstrual period when it was released to the extracellular space from the cytoplasm. The expression of TIMP-1 was not clearly changed during the menstrual cycle. From these results, it was suggested that the destruction of the surrounding matrix by endometriosis might be caused by various MMPs, which are mainly produced in stromal cells.
机译：与子宫内膜异位症一样，子宫内膜异位症具有独特的侵袭和转移特征，尽管从病理学上讲，它是一种良性肿瘤。但是，子宫内膜异位症周围组织破坏的机制仍不清楚。本研究采用免疫组织化学方法检测20例基质金属蛋白酶（MMP）-1，-2，-3，-7，-9和组织抑制剂-1（TIMP-1）的表达和定位，并分析其含量。 ELISA法和Western blotting法检测子宫内膜瘤液中MMP-1，TIMP-1和MMP-1 / TIMP-1复合物的表达，免疫组化分析20例。在月经期间，在基质和上皮细胞中均强烈检测到MMP-1，-2和-9，在上皮细胞中均检测到MMP-7。 MMP-3主要在含有含铁血黄素的巨噬细胞中表达，但表达的变化尚不清楚。在月经期在基质细胞和上皮细胞中都强烈检测到TIMP-1，但在月经周期的其他阶段表达下降。 MMP-1的ELISA也显示出与免疫组织化学相似的结果，这表明它在月经期从细胞质释放到细胞外空间时在月经期释放到囊肿中。在月经周期中，TIMP-1的表达没有明显改变。从这些结果表明，子宫内膜异位对周围基质的破坏可能是由多种MMP引起的，这些MMP主要在基质细胞中产生。

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