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首页> 外文期刊>Life sciences >DUAL COUPLING AND DOWN REGULATION OF HUMAN FSH RECEPTOR IN CHO CELLS
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DUAL COUPLING AND DOWN REGULATION OF HUMAN FSH RECEPTOR IN CHO CELLS

机译：CHO细胞中人FSH受体的双重偶联和下调

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The FSH receptor is a member of the family of G protein-coupled receptors that activate adenylyl cyclase. The binding of agonist to cell surface receptors leads to a reduction in the intensity of the response to continuous stimulation, a process that is usually referred to as desensitization. Although the exact mechanism is not fully understood, the molecular cloning of the FSH receptor has made it possible to study desensitization in transfected cell lines. In this experiment FSH-induced desensitization was studied using Chinese hamster ovary cells expressing a functional human FSH receptor (CHO-FSHR cells). Stimulation of the CHO-FSHR cells with 10 ng/ml human FSH resulted in a decreased sensitivity to a second FSH stimulation. This decrease in FSH-induced cAMP production was observed within 2 h, and exposure of cells to FSH for 20 h led to a 70-80 % inhibition of cAMP formation. Moreover, the desensitization effect observed in CHO cells was mimicked by forskolin and, therefore, was mediated by cAMP. Incubation of cells with I-125-FSH showed an efficient internalization of the ligand in the CHO-FSHR cells. The CHO-FSHR cells rapidly internalized approximately 30% of the receptor-associated I-125-FSH by 2 h and 50% by 4 h. The responsiveness of individual CHO-FSHR cells to FSH was studied and administration of human FSH(30 ng/ml) induced a rapid rise in cytosolic calcium, reaching a peak at 6 sec. The data that human FSH can increase intracellular calcium in cells transfected with the FSH receptor cDNA reveal the possibility for the human FSH receptor to couple to both adenylyl cyclase and phospholipase C cascades. [References: 19]
机译：FSH受体是激活腺苷酸环化酶的G蛋白偶联受体家族的成员。激动剂与细胞表面受体的结合导致对连续刺激的反应强度降低，该过程通常称为脱敏。尽管尚未完全了解确切的机制，但FSH受体的分子克隆已使研究转染细胞系的脱敏成为可能。在该实验中，使用表达功能性人FSH受体的中国仓鼠卵巢细胞（CHO-FSHR细胞）研究了FSH诱导的脱敏。用10 ng / ml人FSH刺激CHO-FSHR细胞会导致对第二次FSH刺激的敏感性降低。在2小时内观察到FSH诱导的cAMP产生的这种减少，并且将细胞暴露于FSH 20 h导致cAMP形成的抑制率为70-80％。而且，在CHO细胞中观察到的脱敏作用被毛喉素模仿，因此被cAMP介导。用I-125-FSH孵育细胞显示CHO-FSHR细胞中配体的有效内在化。 CHO-FSHR细胞在2小时内迅速内化了约30％的受体相关I-125-FSH，在4小时内迅速内化了50％。研究了单个CHO-FSHR细胞对FSH的反应性，施用人FSH（30 ng / ml）诱导了胞质钙的快速升高，在6秒时达到峰值。人类FSH可以增加被FSH受体cDNA转染的细胞中细胞内钙的数据，揭示了人类FSH受体可能同时与腺苷酸环化酶和磷脂酶C级联偶合。 [参考：19]

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