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首页> 外文期刊>Life sciences >Pituitary adenylate cyclase-activating polypeptide (PACAP) 38 and PACAP27 differentially stimulate growth hormone release and mRNA accumulation in porcine somatotropes.
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Pituitary adenylate cyclase-activating polypeptide (PACAP) 38 and PACAP27 differentially stimulate growth hormone release and mRNA accumulation in porcine somatotropes.

机译：垂体腺苷酸环化酶激活多肽（PACAP）38和PACAP27差异刺激猪生长激素中生长激素的释放和mRNA的积累。

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摘要

Pituitary adenylate cyclase-activating polypeptide (PACAP) has been suggested to regulate growth hormone (GH) secretion in several species. Here, we analyzed the in vitro effects of PACAP38 and PACAP27 on the secretory activity of porcine somatotropes. Cultures of porcine pituitary cells were treated with PACAP38 and PACAP27, and GH release, intracellular GH content, and GH mRNA levels were evaluated. Also, the time course of changes in the somatotrope content of GH and its mRNA in response to PACAPs were measured. Both PACAPs stimulated GH release from porcine somatotropes in a broad range of doses (10(-10)-10(-6) M), yet only PACAP27 elicited a dose-dependent response. GH cell content remained essentially unchanged after PACAP treatment. In contrast, both PACAPs induced significant and sustained increases in GH mRNA cell content, although the response to PACAP27 appeared faster (8 h) than to PACAP38 (16 h). These results demonstrate that PACAP stimulates GH production in porcine somatotropes. Furthermore, the differential responses induced by PACAP38 and PACAP27 suggest that distinct mechanisms mediate their effects on this cell type.
机译：已建议垂体腺苷酸环化酶激活多肽（PACAP）调节几种物种的生长激素（GH）分泌。在这里，我们分析了PACAP38和PACAP27对猪生长激素分泌活性的体外作用。用PACAP38和PACAP27处理猪垂体细胞培养物，并评估GH释放，细胞内GH含量和GH mRNA水平。同样，测量了GH的生长激素含量及其mRNA响应PACAP而变化的时间过程。两种PACAPs均以较宽的剂量范围（10（-10）-10（-6）M）刺激猪生长激素释放GH，但只有PACAP27引起剂量依赖性反应。 PACAP处理后，GH细胞含量基本保持不变。相反，尽管对PACAP27的反应（8小时）比对PACAP38的反应（16小时）更快，但两个PACAP都诱导GH mRNA细胞含量显着且持续增加。这些结果证明PACAP刺激猪生长激素中GH的产生。此外，PACAP38和PACAP27诱导的差异反应表明，不同的机制介导了它们对这种细胞类型的作用。

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