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首页> 外文期刊>Life sciences >Variations of prostaglandin E2 receptors in hamster's ovary and endometrium during estrous cycle.
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Variations of prostaglandin E2 receptors in hamster's ovary and endometrium during estrous cycle.

机译：动情周期中仓鼠卵巢和子宫内膜中前列腺素E2受体的变化。

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The purpose of this study was to determine concurrent ovarian and endometrial prostaglandin E2 (PGE2) receptor concentrations throughout the hamster estrous cycle. The effect of progesterone (P4) on PGE2 receptor in these two tissues was also investigated during in vitro culture. Estrous cycles of mature female hamsters were monitored according to the appearance of the vaginal discharge on cycle day 1 (D1). Ovaries and uteri were removed from cyclic hamsters at 10:00 a.m. on each day of the cycle and at 6:00 p.m. on D4 (proestrus). Ovarian and endometrial cell membranes were collected and assayed for the specific PGE2 binding by Scatchard plot analysis, using seven different concentrations of 3H-PGE2 (0.72-9.1 nM) with or without the presence of unlabelled PGE2 (9.1 microM). Ovarian and endometrial tissues of the cyclic hamsters were shown to contain a saturable, specific binding site with KD=4.69+/-0.55, and 5.7+/-0.4 nM for ovary and endometrium, respectively. Relative binding activity of PGE1 for the PGE2 binding site was about 28%. PGF2alpha and PGA1 did not compete for the PGE2 binding site. In ovaries, the PGE2 receptor levels started to increase sharply in the evening of D4 and reached maximum in the morning of D1. A precipitous drop of PGE2 receptor was observed on D2 followed by gradual decreases on D3 and D4. The PGE2 receptor concentrations in endometrium were the lowest in the morning of D4, and increased thereafter until a maximal level was reached on D2. Progesterone (10 nM) augmented PGE2 receptors in ovarian but not in endometrial tissue during 24-h in vitro incubation.
机译：这项研究的目的是确定整个仓鼠发情周期中同时存在的卵巢和子宫内膜前列腺素E2（PGE2）受体的浓度。在体外培养过程中，还研究了孕酮（P4）对这两个组织中PGE2受体的影响。根据第1天（D1）白带的出现情况，监测成熟雌性仓鼠的发情周期。在周期的每一天的上午10:00和下午6:00，从周期性仓鼠中取出卵巢和子宫。在D4（发情期）。收集卵巢和子宫内膜细胞膜，并通过Scatchard图分析，使用七种不同浓度的3H-PGE2（0.72-9.1 nM），存在或不存在未标记PGE2（9.1 microM）的情况下，通过Scatchard图分析来测定特异性PGE2结合。环状仓鼠的卵巢和子宫内膜组织显示含有可饱和的特异性结合位点，其卵巢和子宫内膜的KD分别为4.69 +/- 0.55和5.7 +/- 0.4 nM。 PGE1对PGE2结合位点的相对结合活性为约28％。 PGF2alpha和PGA1不竞争PGE2结合位点。在卵巢中，PGE2受体水平在D4晚上开始急剧增加，并在D1早晨达到最高。在D2上观察到PGE2受体急剧下降，然后在D3和D4上逐渐下降。子宫内膜中的PGE2受体浓度在D4早晨最低，然后增加直到D2达到最高水平。在体外培养24小时期间，孕酮（10 nM）在卵巢中增加PGE2受体，但在子宫内膜组织中不增加。

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