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首页> 外文期刊>Life sciences >Reduced food consumption increases water intake and modulates renal aquaporin-1 and -2 expression in autoimmune prone mice.
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Reduced food consumption increases water intake and modulates renal aquaporin-1 and -2 expression in autoimmune prone mice.

机译：食物消耗量的减少会增加自身免疫易发小鼠的水摄入量，并调节肾脏aquaporin-1和-2的表达。

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Aquaporin-1(AQP1) and AQP2 are members of the aquaporin family of cell membrane water channel transport proteins and have been implicated in the regulation of renal water excretion. We have previously shown that calorie restriction (CR) relative to ad libitum (AL) feeding extends lifespan and delays the onset of autoimmune kidney disease in lupus-prone (NZBxNZW)F1 (B/W) mice. To determine if AQP1 and/or AQP2 expression is influenced by CR, mice were fed an AL or CR (40% less food) diet until 4 (young) or 9 (old) months of age when mice were sacrificed. Kidneys were removed and the expression of AQP1 and AQP2 was determined at the protein and mRNA levels using western blotting and RT-PCR respectively. While age did not significantly increase AQP1 expression in the AL groups, CR did increase both the protein (1.4-fold) and mRNA (2.4-fold) levels. In old mice, AQP1 expression was higher (1.8-fold) in CR compared to the AL group while CR had no effect in young mice. In contrast, AQP2 showed an age related decrease (55%) in the AL groups and an increase in the protein (8.4-fold) and mRNA (1.7-fold) levels in the CR groups. Relative to AL, CR decreased AQP2 expression at the protein (90%) and mRNA (50%) levels in the young mice while an increase at the protein (2.9-fold) and mRNA (1.9-fold) levels was evident in the old mice. Interestingly, a significant increase in water intake per gram body weight was found in both young and old CR fed mice when compared to their AL counterparts which may contribute to the prevention of autoimmune disease with age and differences in longevity. These data show, for the first time, significant age and diet influences in renal AQP1 and AQP2 expression at both protein and mRNA levels in lupus-prone mice.
机译：Aquaporin-1（AQP1）和AQP2是细胞膜水通道转运蛋白水通道蛋白家族的成员，并参与了肾脏水排泄的调节。先前我们已经证明，相对于随意喂养（AL）的卡路里限制（CR）可以延长易发狼疮（NZBxNZW）F1（B / W）小鼠的寿命并延迟自身免疫性肾脏疾病的发作。为了确定AQP1和/或AQP2的表达是否受CR影响，对小鼠进行AL或CR（少40％的食物）饮食，直到处死小鼠的年龄为4（年轻）或9（大）个月。去除肾脏，分别使用蛋白质印迹和RT-PCR在蛋白和mRNA水平上测定AQP1和AQP2的表达。虽然年龄没有显着增加AL组中AQP1的表达，但CR确实增加了蛋白质（1.4倍）和mRNA（2.4倍）水平。在老年小鼠中，与AL组相比，CR中的AQP1表达更高（1.8倍），而CR在年轻小鼠中没有作用。相反，AQP2在AL组中显示出与年龄相关的下降（55％），在CR组中显示出蛋白质水平（8.4倍）和mRNA（1.7倍）的增加。相对于AL，CR降低了幼鼠的蛋白质（90％）和mRNA（50％）水平的AQP2表达，而老龄小鼠的蛋白质（2.9倍）和mRNA（1.9倍）水平明显升高老鼠。有趣的是，年轻和年老的CR喂养小鼠与AL小鼠相比，每克体重的摄水量显着增加，这可能有助于预防年龄和寿命差异的自身免疫性疾病。这些数据首次显示了易发狼疮小鼠的年龄和饮食对肾脏AQP1和AQP2表达在蛋白质和mRNA水平上的影响。

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