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首页> 外文期刊>Life sciences >The MEF2 site is necessary for induction of the myosin light chain 2 slow promoter in overloaded regenerating plantaris muscle.
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The MEF2 site is necessary for induction of the myosin light chain 2 slow promoter in overloaded regenerating plantaris muscle.

机译：在过载的再生plant肌中，MEF2位点是诱导肌球蛋白轻链2慢启动子所必需的。

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摘要

Functional overload (OV) of the rat plantaris muscle results in a fast to slow change in muscle phenotype with induction of the slow contractile protein genes including myosin light chain 2 slow (MLC2s). To identify potential cis-acting DNA sites regulating MLC2s following ablation, plasmid constructs were transfected in vivo into regenerating overloaded plantaris muscles. Activity of the 270bp promoter (-270MLC2s) was increased in OV muscles at 28 days. Mutation of the MEF2 site (-270MEF2) knocked out the overload-induced activity of the promoter. Mutation of the Ebox (-270Ebox) resulted in an earlier induction with OV and mutation of the CACC site (-270CACC) resulted in increased activity in the CON PLN with OV induction detected by 21 days. These results demonstrate that the -270MLC2s promoter contains the elements necessary for expression of MLC2s in regenerating OV PLN. More importantly, mutation analysis of -270MLC2s promoter demonstrates that mechanical loading induced expression shares some common molecular mechanisms with slow nerve dependent model regulation. In these two models of physiological induction of MLC2s, the CACC site acts as a repressor region (on/off switch) and the MEF2 site acts to modulate quantitative expression.
机译：大鼠plant肌的功能超负荷（OV）导致肌肉表型的快慢变化，其中包括肌球蛋白轻链2慢（MLC2s）的慢收缩蛋白基因的诱导。为了鉴定消融后调节MLC2的潜在顺式作用DNA位点，将质粒构建体体内转染到再生的超负荷plant肌中。在28天时，OV肌肉中270bp启动子（-270MLC2s）的活性增加。 MEF2位点（-270MEF2）的突变敲除了启动子的超负荷诱导活性。 Ebox（-270Ebox）突变导致更早的OV诱导，CACC位点（-270CACC）突变导致CON PLN中的活性增加，到21天检测到OV诱导。这些结果证明-270MLC2s启动子包含在再生OV PLN中表达MLC2s所必需的元件。更重要的是，对-270MLC2s启动子的突变分析表明，机械加载诱导的表达与慢神经依赖性模型调控共享一些共同的分子机制。在这两种MLC2生理诱导模型中，CACC位点充当阻遏物区域（开/关开关），而MEF2位点充当调节定量表达的作用。

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