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首页> 外文期刊>Life sciences >Heyneanol A induces apoptosis via cytochrome c release and caspase activation in human leukemic U937 cells
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Heyneanol A induces apoptosis via cytochrome c release and caspase activation in human leukemic U937 cells

机译：Heyneanol A通过人白血病U937细胞中的细胞色素c释放和caspase激活诱导凋亡

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Heyncanol A, a tetramer of resveratrol, is isolated from the roots of Vitis amurensis by cytotoxicity based fractionation. In this study, the mechanism of apoptosis by heyneanol A was evaluated in human leukemic U937 cells. Heyneanol A (IC50 = 6.6 muM at 24 h) exhibited stronger cytotoxic effect than resveratrol (IC50 = 100 muM at 24 h) by 15-fold on human leukemic U937 cells by XTT assay. Apoptotic bodies were observed in U937 cells treated with 6 muM of heyneanol A by TUNEL assay. Heyneanol A effectively increased the portion of sub-G, DNA content in a time- and concentration-dependent manner by flow cytometric analysis. Heyneanol A also induced cytochrome c release from mitochondria into the cytosol and subsequent caspase activation involving caspase 9 and 3 to cleave PARR However, it did not affect the expressions of Bax and Bcl-2 by western blotting. It was confirmed that the activation of caspase 8, 9 and 3 and the cleavage of PAR-P by heyneanol A were completely blocked by adding Z-VAD-FMK, a caspase inhibitor. These findings suggest that heyneanol A has anti-tumor activity, which may be mediated by apoptosis caused by cytochrome c release and caspase activation in human leukemic U937 cells. (C) 2004 Elsevier Inc. All rights reserved. [References: 34]
机译：通过基于细胞毒性的分级分离，从白葡萄的根中分离出白藜芦醇的四聚体海英醇A。在这项研究中，在人白血病U937细胞中评估了炔丙醇A凋亡的机制。通过XTT分析，对人白血病U937细胞，Heyneanol A（24 h时IC50 = 6.6μM）表现出比白藜芦醇（24 h时IC50 = 100μM）更强的细胞毒性作用。通过TUNEL测定，在用6μM的Heyneanol A处理的U937细胞中观察到凋亡小体。 Heyneanol A通过流式细胞仪分析以时间和浓度依赖性方式有效地增加了亚G部分，DNA含量。 Heyneanol A还诱导细胞色素c从线粒体释放到细胞质中，随后caspase活化涉及caspase 9和3裂解PARR，但是，它不会通过Western blotting影响Bax和Bcl-2的表达。已经证实，通过添加胱天蛋白酶抑制剂Z-VAD-FMK，胱天蛋白酶8、9和3的活化以及肝烯醇A对PAR-P的切割被完全阻断。这些发现表明，heyneanol A具有抗肿瘤活性，这可能是由人类白血病U937细胞中细胞色素c释放和胱天蛋白酶激活引起的凋亡介导的。 （C）2004 Elsevier Inc.保留所有权利。 [参考：34]

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