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首页> 外文期刊>Life sciences >Antibacterial activity of ethanolic and aqueous extracts of Acacia aroma Gill. ex Hook et Arn.
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Antibacterial activity of ethanolic and aqueous extracts of Acacia aroma Gill. ex Hook et Arn.

机译：金合欢香G的乙醇和水提取物的抗菌活性。前胡克等

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摘要

The purpose of the present study was to investigate the antibacterial activity of seven ethanolic extracts and three aqueous extracts from various parts (leaves, stems and flowers) of A. aroma against 163 strains of antibiotic multi-resistant bacteria. The disc diffusion assay was performed to evaluate antibacterial activity of the A. aroma crude extracts, against several Gram-positive bacteria (E. faecalis, S. aureus, coagulase-negative stahylococci, S. pyogenes, S. agalactiae, S. aureus ATCC 29213, E. faecalis ATCC 29212) and Gram-negative bacteria (E. coli., K. pneumoniae, P. mirabilis, E. cloacae, S. marcescens, M morganii, A. baumannii, P. aeruginosa, S. maltophilia, E. coli ATCC 35218, P. aeruginosa ATCC 27853, E. coli ATCC 25922). All ethanolic extracts showed activity against gram-positive bacteria. Among all obtained extracts, only leaf and flower fluid extracts showed activity against Gram-negative bacteria. Based on this bioassay, leaf fluid extracts tended to be the most potent, followed byflower fluid extracts. Minimal inhibitory concentration (MIC) values of extracts and antibiotics were comparatively determined by agar and broth dilution methods. Both extracts were active against S. aureus, coagulase-negative stahylococci, E. faecalis and E. faecium and all tested Gram-negative bacteria with MIC values from 0.067 to 0.308 mg/ml. In this study the minimal bactericidal concentration (MBC) values were identical or twice as high than the corresponding MIC for leaf extracts and four or eight times higher than MIC values for flower extracts. This may indicate a bactericidal effect. Stored extracts have similar antibacterial activity as recently obtained extracts. The A. aroma extracts of leaves and flowers may be useful as antibacterial agents against Gram- negative and Gram-positive antibiotic multi-resistant microorganisms.
机译：本研究的目的是研究7种乙醇提取物和3种来自A.aroma香气各个部位（叶，茎和花）的水提取物对163株抗生素多重耐药菌的抗菌活性。进行椎间盘扩散测定以评估曲霉粗提物对几种革兰氏阳性细菌（粪肠球菌，金黄色葡萄球菌，凝固酶阴性葡萄球菌，化脓性链球菌，无乳链球菌，金黄色葡萄球菌ATCC）的抗菌活性。 29213，粪肠球菌（E. faecalis ATCC 29212）和革兰氏阴性细菌（大肠杆菌，肺炎克雷伯氏菌，奇异毕赤酵母，阴沟肠杆菌，marcescens，摩根氏菌，鲍曼不动杆菌，铜绿假单胞菌，嗜麦芽糖杆菌大肠杆菌ATCC 35218，铜绿假单胞菌ATCC 27853，大肠杆菌ATCC 25922）。所有乙醇提取物均具有抗革兰氏阳性细菌的活性。在所有获得的提取物中，只有叶和花液提取物显示出对革兰氏阴性细菌的活性。基于该生物测定法，叶液提取物往往是最有效的，其次是花液提取物。通过琼脂和肉汤稀释法比较确定提取物和抗生素的最低抑菌浓度（MIC）值。两种提取物均对金黄色葡萄球菌，凝固酶阴性葡萄球菌，粪肠球菌和粪肠球菌以及所有测试的革兰氏阴性菌具有活性，其MIC值为0.067至0.308 mg / ml。在这项研究中，最低的杀菌浓度（MBC）值与叶提取物的相应MIC相同或高出两倍，而对花提取物的MIC值则高出四到八倍。这可能表明有杀菌作用。储存的提取物具有与最近获得的提取物相似的抗菌活性。叶子和花朵的香气提取物可用作对抗革兰氏阴性和革兰氏阳性抗生素多重耐药微生物的抗菌剂。

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