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Expression and hormonal regulation of calcyclin-binding protein (CacyBP) in the mouse uterus during early pregnancy

机译：孕早期小鼠子宫中钙调蛋白结合蛋白（CacyBP）的表达及其激素调控

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摘要

Calcyclin-binding protein (Siah-1-Interacting Protein, CacyBP/SIP), is a calcium signaling protein involved in the degradation of p-catenin, however, little is known about its role in reproductive biology. The present study was to character its temporospatial expression pattern and regulation in mouse uterus and to investigate whether it plays a role in the regulation of normal endometrial events. While prominently expressed in both luminal and glandular epithelia, CacyBP underwent dynamic changes during early pregnancy. CacyBP expression was observed weakly from days 1-4. An intense accumulation in luminal and glandular epithelia as well as decidua surrounding the embryo at later stages (days 5-7) was observed. Most notably, CacyBP accumulation in trophoblast was pronounced at day 7. Using ovariectomized and pseudopregnant mice, we found that progesterone (P-4) and l7 beta-estradiol (E-2) led to increased expression of CacyBP gene and this could be abolished by Ru486 and tamoxifen, respectively. Antisense oligonucleotides (ODNs) against CacyBP significantly inhibited cultured endometrial stromal cells' (ESCs) apoptosis induced by UV irradiation. Injection of antisense ODNs into mouse uterine horn severely impaired the number of implanted blastocysts. Taken together, our results suggested that CacyBP expression was positively regulated by P4 and E,. CacyBP may be involved in the regulation of endometrial cell apoptosis during early pregnancy and play an important role in mouse endometrial events such as pregrancy establishment. (c) 2005 Elsevier Inc. All rights reserved.
机译：钙调蛋白结合蛋白（Siah-1-Interacting蛋白，CacyBP / SIP）是一种钙信号蛋白，参与p-catenin的降解，但是，其在生殖生物学中的作用鲜为人知。本研究旨在表征其在小鼠子宫中的颞pat表达模式和调控，并研究其是否在正常子宫内膜事件的调控中起作用。虽然在腔和腺上皮细胞中均显着表达，但CacyBP在妊娠早期发生了动态变化。从1-4天开始微弱地观察到CacyBP表达。在后期（第5-7天）观察到了腔和腺上皮以及蜕膜周围的蜕膜大量积累。最值得注意的是，在第7天，滋养细胞中的CacyBP蓄积明显。使用去卵巢和假孕小鼠，我们发现黄体酮（P-4）和17β-雌二醇（E-2）导致CacyBP基因表达增加，可以消除这种情况。分别由Ru486和他莫昔芬。针对CacyBP的反义寡核苷酸（ODNs）显着抑制了UV辐射诱导的培养的子宫内膜基质细胞（ESCs）凋亡。向小鼠子宫角注射反义ODNs严重损害了植入的胚泡的数量。两者合计，我们的结果表明，CacyBP表达受到P4和E，正调控。 CacyBP可能参与早期妊娠期间子宫内膜细胞凋亡的调控，并在小鼠子宫内膜事件（如建立妊娠）中发挥重要作用。 （c）2005 Elsevier Inc.保留所有权利。

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