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Rat arteries contain multiple nicotinic acetylcholine receptor alpha-subunits.

机译:大鼠动脉包含多个烟碱型乙酰胆碱受体α亚基。

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摘要

We investigated the occurrence and distribution of the ligand-binding alpha-subunits of nicotinic acetylcholine receptors in the rat arterial system in situ by means of RT-PCR and immunohistochemistry. Except the alpha9-subunit, all other mammalian non-muscular alpha-subunits were expressed in the arterial wall - either in endothelial or in smooth muscle cells - suggesting it as a direct target of nicotine and endogenous acetylcholine. The distribution pattern of alpha-subunits found in smooth muscle cells varied considerably among the individual elastic, muscular and intraparenchymal arteries investigated, suggesting that non-neuronal cholinergic signalling via nicotinic receptors in the vascular wall includes components that are highly specific for individual arteries.
机译:我们通过RT-PCR和免疫组织化学方法研究了大鼠动脉系统中烟碱乙酰胆碱受体配体结合α-亚基的发生和分布。除α9亚基外,所有其他哺乳动物非肌肉α亚基均在动脉壁上表达-在内皮或平滑肌细胞中-提示它是尼古丁和内源性乙酰胆碱的直接靶标。在研究的单个弹性,肌肉和实质内动脉之间,在平滑肌细胞中发现的α-亚基的分布模式有很大差异,这表明通过烟碱样受体在血管壁中产生的非神经元胆碱能信号包括对单个动脉高度特异性的成分。

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