• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >Differential effects of diabetes induced by streptozotocin and that develops spontaneously on prostate growth in Bio Breeding (BB) rats.
【24h】

Differential effects of diabetes induced by streptozotocin and that develops spontaneously on prostate growth in Bio Breeding (BB) rats.

机译：链脲佐菌素诱发的糖尿病的差异作用，并自发地对生物育种（BB）大鼠的前列腺生长产生影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We investigated molecular changes in the response to insulin in prostates of spontaneously developed (Bio Breeding) and streptozotocin (STZ)-induced diabetic rats that received sufficient amounts (euglycemic group), or suboptimal doses (hyperglycemic group) of insulin for 32 weeks, using Affymetrix GeneChip analysis of gene expression. Alterations in gene expression levels identified by microarray analysis, having potential biological relevance to prostate growth, were verified by real-time reverse transcription polymerase chain reaction (RT-PCR). A significant decrease in the weight of ventral prostate was observed in the hyperglycemic STZ-induced but not spontaneously developed diabetic group. Microarray analysis revealed that gene expression profiles were distinctly different in each region of the prostate, and that hyperglycemic diabetes in spontaneously developed and STZ-diabetic rats was associated with differential changes in the prostatic expression levels of 856 genes, of which 35 were relatedto cell growth, proliferation and death. RT-PCR data verified significant differences in the mRNA expression levels of Igfbp6, Tieg, and Clu between euglycemic and hyperglycemic groups, whereas expression levels of these genes in control and euglycemic diabetic groups were not significantly different. In ventral prostate, the mRNA expression levels of Igfbp6 and Tieg were significantly higher in the hyperglycemic STZ-induced diabetic than in the hyperglycemic spontaneously diabetic BBDP/Wor rats. Our data demonstrate that the diabetes induced by STZ in the BBDR/Wor rats affects prostate growth and the molecular response to insulin differently than that observed in BBDP/Wor rats that develop diabetes spontaneously.
机译：我们调查了接受自发（生物育种）和链脲佐菌素（STZ）诱导的糖尿病大鼠的前列腺对胰岛素反应的分子变化，这些大鼠接受了足量（正常血糖组）或次适量剂量（高血糖组）持续32周的胰岛素，使用Affymetrix基因表达分析。通过实时逆转录聚合酶链反应（RT-PCR）验证了通过微阵列分析确定的与前列腺生长具有潜在生物学相关性的基因表达水平的变化。在高血糖STZ诱导但非自发发展的糖尿病组中观察到腹侧前列腺重量的显着降低。基因芯片分析显示，前列腺各区域的基因表达谱明显不同，自发性发展和STZ-糖尿病大鼠的高血糖糖尿病与856个基因的前列腺表达水平的差异有关，其中35个与细胞生长有关，扩散和死亡。 RT-PCR数据证实了正常血糖组和高血糖组之间Igfbp6，Tieg和Clu的mRNA表达水平有显着差异，而对照组和正常血糖糖尿病组中这些基因的表达水平没有显着差异。在高血糖自发性糖尿病BBDP / Wor大鼠中，在高血糖STZ诱导的糖尿病患者中，腹侧前列腺中Igfbp6和Tieg的mRNA表达水平显着更高。我们的数据表明，与自发性糖尿病的BBDP / Wor大鼠相比，BBDR / Wor大鼠由STZ诱导的糖尿病影响前列腺生长和对胰岛素的分子反应不同。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号