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首页> 外文期刊>Life sciences >THE ANTIGENIC SITES OF TRICHOSANTHIN, A RIBOSOME-INACTIVATING PROTEIN WITH MULTIPLE PHARMACOLOGICAL PROPERTIES
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THE ANTIGENIC SITES OF TRICHOSANTHIN, A RIBOSOME-INACTIVATING PROTEIN WITH MULTIPLE PHARMACOLOGICAL PROPERTIES

机译：具有多种药理特性的核糖体失活蛋白天花粉蛋白的抗原性位点

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Trichosanthin (TCS) fragments were produced by Tn1000 deletion mutagenesis and by cyanogen bromide (CNBr) cleavage and their immunoreactivity was examined by incubating with various antibodies. Twelve C-terminally truncated TCS variants were successfully synthesized under the control of a T7 RNA driven promoter. The smallest antigenic fragment mapped corresponded to the N-terminal 20 amino acids (aa). Six CNBr fragments of TCS were created and identified by electrospray mass spectrometry and N-terminal sequencing, Three antigenic fragments corresponding to aa 1-72, 101-152 and 153-246, respectively were mapped. Fragments corresponding to aa 1-72 and 153-246 were immunoreactive to the same monoclonal antibody showing they are components of a discontinuous epitope. On the other hand, the fragment containing aa 73-100 was not detected by any of the antibodies used. [References: 34]
机译：天花粉蛋白（TCS）片段是通过Tn1000缺失诱变和溴化氰（CNBr）切割产生的，并通过与各种抗体孵育来检查其免疫反应性。在T7 RNA驱动的启动子的控制下，成功合成了十二个C端截短的TCS变体。映射的最小抗原片段对应于N端20个氨基酸（aa）。创建了六个TCS的CNBr片段，并通过电喷雾质谱和N端测序进行了鉴定，分别绘制了对应于氨基酸1-72、101-152和153-246的三个抗原性片段。对应于氨基酸1-72和153-246的片段对同一单克隆抗体具有免疫反应性，显示它们是不连续表位的组成部分。另一方面，所用的任何抗体均未检测到含有aa 73-100的片段。 [参考：34]

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