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首页> 外文期刊>Life sciences >MICRODIALYSIS AS A TOOL TO MEASURE DIETARY AND REGIONAL EFFECTS ON THE COMPLETE PROFILE OF EXTRACELLULAR AMINO ACIDS IN THE HYPOTHALAMUS OF RATS
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MICRODIALYSIS AS A TOOL TO MEASURE DIETARY AND REGIONAL EFFECTS ON THE COMPLETE PROFILE OF EXTRACELLULAR AMINO ACIDS IN THE HYPOTHALAMUS OF RATS

机译：微量透析作为测量大鼠下丘脑细胞外氨基酸完整轮廓的饮食和区域影响的工具

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Regional and dietary-induced changes in hypothalamic extracellular amino acid concentrations were examined. Microdialysis probes were simultaneously implanted in the hypothalamic paraventricular nucleus (PVN) and the lateral hypothalamus (LH) of anesthetized rats and perfused at a rate of 2 mu l/min. Dialysates were collected every 20 min for 1 h prior to gavage of a balanced amino acid mixture (0.85 g patterned after 1 g of chicken egg albumin) and then every 20 min for 3 h after treatment. Tail vein blood samples were also collected. Marked changes in plasma levels of most amino acids were evident immediately following the amino acid gavage. In the PVN, concentrations of isoleucine, leucine, methionine and valine all increased within 40 min, whereas significant decreases in glutamine, histidine and taurine were observed in the LH. In a separate study, PVN extracellular amino acid concentrations were examined in awake, freely-behaving rats following gavage of equicaloric loads of a balanced amino acid mixture, glucose (0.89 g) or water. Dialysate levels of glutamate, isoleucine, leucine, methionine, threonine, tyrosine and valine showed reliable increases after amino acid treatment, although the overall time course of these effects differed somewhat. The amino acid profile of the PVN was, in general, unaffected by glucose administration. These findings suggest that specific brain regions may respond uniquely to amino acid ingestion and further imply that dietary composition may influence the amino acid profiles of the extracellular fluid in brain. [References: 29]
机译：检查了下丘脑细胞外氨基酸浓度的区域性和饮食性变化。将微透析探针同时植入麻醉大鼠的下丘脑室旁核（PVN）和下丘脑外侧（LH），并以2μl/ min的速度灌注。在管饲平衡氨基酸混合物（每1克鸡蛋清蛋白1克后形成0.85克）之前，每20分钟收集透析液1小时，然后在治疗后每20分钟收集3小时。还收集了尾静脉血液样本。氨基酸管饲后，大多数氨基酸的血浆水平明显变化。在PVN中，异亮氨酸，亮氨酸，蛋氨酸和缬氨酸的浓度在40分钟内均增加，而在LH中观察到谷氨酰胺，组氨酸和牛磺酸显着降低。在另一项研究中，在摄入均衡卡路里混合物，葡萄糖（0.89 g）或水的等热量负荷后，在清醒自由行为的大鼠中检查了PVN细胞外氨基酸的浓度。氨基酸处理后，谷氨酸，异亮氨酸，亮氨酸，蛋氨酸，苏氨酸，酪氨酸和缬氨酸的透析液水平显示出可靠的增加，尽管这些作用的总时间过程有所不同。通常，PVN的氨基酸谱不受葡萄糖施用的影响。这些发现表明特定的大脑区域可能对氨基酸摄入有独特的反应，并进一步暗示饮食结构可能会影响大脑中细胞外液的氨基酸谱。 [参考：29]

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