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首页> 外文期刊>Life sciences >Regulations by cyclic nucleotides and phorbol ester of calcium efflux from cultured bovine adrenal chromaffin cells.
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Regulations by cyclic nucleotides and phorbol ester of calcium efflux from cultured bovine adrenal chromaffin cells.

机译：由培养的牛肾上腺嗜铬细胞的钙外流的环状核苷酸和佛波酯调节。

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摘要

The effects of cyclic nucleotides and phorbol ester on Ca2+ efflux from cultured bovine adrenal chromaffin cells were examined. Dibutyryl cyclic AMP (DB-cAMP), forskolin (an activator of adenylate cyclase), dibutyryl cyclic GMP (DB-cGMP) and nitroprusside (an activator of guanylate cyclase) all stimulated 45Ca2+ efflux from the cells preloaded with 45Ca2+. These agents did not increase the intracellular free Ca2+ ([Ca2+]i) level. On the contrary, phorbol 12-myristate 13-acetate (PMA; an activator of protein kinase C) did not affect the efflux of 45Ca2+, but inhibited the increase in 45Ca2+ efflux caused by DB-cAMP, forskolin, DB-cGMP or nitroprusside. The 45Ca2+ effluxes stimulated by cyclic nucleotides, forskolin and nitroprusside were inhibited by deprivation of extracellular Na+ ([Na+]o). These results suggest that both cAMP- and cGMP-dependent protein kinases are involved in the stimulatory mechanism of [Na+]o dependent Ca2+ efflux, probably through acceleration of [Na+]o/[Ca2+]i exchange and that protein kinase C plays an inhibitory role in this mechanism.
机译：研究了环核苷酸和佛波酯对培养的牛肾上腺嗜铬细胞钙离子外流的影响。二丁酰环AMP（DB-cAMP），福司可林（腺苷酸环化酶的激活剂），二丁酰环GMP（DB-cGMP）和硝普钠（鸟苷酸环化酶的激活剂）均刺激了预装有45Ca2 +的细胞的45Ca2 +外排。这些药物不会增加细胞内游离Ca2 +（[Ca2 +] i）的水平。相反，佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA;蛋白激酶C的激活剂）不影响45Ca2 +的外排，但抑制了由DB-cAMP，毛喉素，DB-cGMP或硝普钠引起的45Ca2 +外排的增加。环核苷酸，毛喉素和硝普钠刺激的45Ca2 +流出被细胞外Na +（[Na +] o）剥夺抑制。这些结果表明，cAMP依赖性蛋白激酶和cGMP依赖性蛋白激酶都可能参与了[Na +] o依赖性Ca2 +外排的刺激机制，这可能是通过促进[Na +] o / [Ca2 +] i交换而引起的，并且蛋白激酶C发挥了抑制作用。在这种机制中的作用。

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