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首页> 外文期刊>Life sciences >CALCIUM EFFLUX FROM CULTURED BOVINE ADRENAL CHROMAFFIN CELLS INDUCED BY PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE (PACAP) - POSSIBLE INVOLVEMENT OF AN NA+/CA2+ EXCHANGE MECHANISM
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CALCIUM EFFLUX FROM CULTURED BOVINE ADRENAL CHROMAFFIN CELLS INDUCED BY PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE (PACAP) - POSSIBLE INVOLVEMENT OF AN NA+/CA2+ EXCHANGE MECHANISM

机译：垂体腺苷酸环化酶多肽（PACAP）诱导的牛牛肾上腺嗜铬细胞钙离子流出-可能涉及NA + / CA2 +交换机制

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The effect of pituitary adenylate cyclase-activating polypeptide 1-38 (PACAP(1-38)) on Ca2+ efflux from cultured bovine adrenal chromaffin cells was examined. PACAP(1-38) stimulated the efflux of Ca-45(2+) from the cells in a concentration dependent manner (10(-9) - 10(-7)M). This effect was inhibited by its potent receptor antagonist PACAP(6-38). PACAP(1-38) increased the formation of [H-3]inositol phosphates and cyclic AMP in the cells. Forskolin, an activator of adenylate cyclase, also stimulated the efflux of Ca-45(2+) from the cells. 3-Isobutyl-1-methylxanthine (IBMX), an inhibitor of phosphodiesterase, enhanced PACAP(1-38)-induced Ca-45(2+) efflux from the cells. Phorbol 12-myristate 13-acetate (PMA), an activator of protein kinase C, had no effect on the efflux of Ca-45(2+) from the cells. The increases in Ca-45(2+) efflux induced by PACAP(1-38) and forskolin were reduced by deprivation of extracellular Na+ and the Na+/Ca2+ exchange inhibitor amiloride. In addition, PACAP(1-38) stimulated Na-22(+) influx into the cells, and this action was inhibited by amiloride. These results suggest that PACAP(1-38) stimulates an Na+/Ca2+ exchange mechanism through activation of adenylate cyclase in cultured bovine adrenal chromaffin cells. [References: 18]
机译：垂体腺苷酸环化酶激活多肽1-38（PACAP（1-38））对培养的牛肾上腺嗜铬细胞Ca2 +外排的影响进行了检查。 PACAP（1-38）以浓度依赖性方式（10（-9）-10（-7）M）刺激Ca-45（2+）从细胞中流出。其有效的受体拮抗剂PACAP（6-38）抑制了这一作用。 PACAP（1-38）增加了细胞中[H-3]肌醇磷酸酯和环状AMP的形成。 Forskolin，腺苷酸环化酶的激活剂，还刺激了Ca-45（2+）从细胞中流出。 3-异丁基-1-甲基黄嘌呤（IBMX），磷酸二酯酶的抑制剂，增强了PACAP（1-38）诱导的Ca-45（2+）从细胞中流出。 Phorbol 12-肉豆蔻酸酯13-乙酸酯（PMA），一种蛋白激酶C的激活剂，对Ca-45（2+）从细胞中的流出没有影响。通过剥夺细胞外Na +和Na + / Ca2 +交换抑制剂阿米洛利，减少了PACAP（1-38）和毛喉素诱导的Ca-45（2+）外流的增加。此外，PACAP（1-38）刺激Na-22（+）流入细胞，这种作用被阿米洛利抑制。这些结果表明，PACAP（1-38）通过活化培养的牛肾上腺嗜铬细胞中的腺苷酸环化酶来刺激Na + / Ca2 +交换机制。 [参考：18]

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