• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >EFFECT OF CHRONIC COCAINE ADMINISTRATION ON AMINO ACID UPTAKE IN RAT PLACENTAL MEMBRANE VESICLES
【24h】

EFFECT OF CHRONIC COCAINE ADMINISTRATION ON AMINO ACID UPTAKE IN RAT PLACENTAL MEMBRANE VESICLES

机译：慢性可卡因对大鼠胎盘膜囊泡中氨基酸摄取的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This study evaluated the effects of chronic exposure to cocaine during pregnancy on amino acid uptake in placental membrane vesicles. Pregnant rats received 62 mg/kg of cocaine hydrochloride by intraperitoneal (IF) injection as a divided daily dose on gestation days 8-19 inclusive. Fetal body weights were significantly decreased by 19% in the cocaine group, while placental weights were unchanged. Placental apical membrane vesicles were prepared from control and cocaine-treated animals, and marker enzyme enrichments for alkaline phosphatase and [H-3]-dihydroalprenolol binding did not differ between cocaine and control groups. Rates of uptake (10 sec) of selected radiolabeled amino acids were measured utilizing a rapid filtration technique. Na+-dependent apical membrane [H-3]-glutamine transport (50 mu M) was reduced by 95% (p < 0.05) in cocaine-treated compared to control placentas. Uptake of 50 mu M [H-3]-methyl aminoisobutyric acid (MeAIB) into apical membranes was also decreased by 43% (p < 0.05) in cocaine membranes. Na+-independent [H-3]-arginine transport (10 mu M), however, did not differ between control or cocaine-treated groups. In summary, chronic cocaine administration selectively inhibited the transport of glutamine and MeAIB into apical membrane vesicles, but had minimal effect on arginine transport. We postulate that this diminution in uptake may contribute to the fetal growth retardation noted in our model. [References: 40]
机译：这项研究评估了怀孕期间长期接触可卡因对胎盘膜囊泡氨基酸吸收的影响。妊娠大鼠在妊娠第8-19天（每天）通过腹膜内（IF）注射接受62 mg / kg的可卡因盐酸盐的每日剂量。可卡因组的胎儿体重显着下降了19％，而胎盘重量没有变化。从对照组和可卡因处理的动物制备胎盘顶膜囊泡，可卡因与对照组之间碱性磷酸酶和[H-3]-二氢普萘洛尔结合的标记酶富集没有差异。使用快速过滤技术测量选定的放射性标记氨基酸的摄取速率（10秒）。与对照胎盘相比，可卡因处理后的Na +依赖性根尖膜[H-3]-谷氨酰胺转运（50μM）降低了95％（p <0.05）。可卡因膜对顶膜的50μM [H-3]-甲基氨基异丁酸（MeAIB）吸收也降低了43％（p <0.05）。对照组或可卡因治疗组之间，不依赖Na +的[H-3]-精氨酸转运（10μM）没有区别。总之，长期服用可卡因可选择性抑制谷氨酰胺和MeAIB转运到根尖膜囊泡中，但对精氨酸转运的影响很小。我们假设摄取的减少可能会导致我们模型中提到的胎儿发育迟缓。 [参考：40]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号