• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Life sciences >ASTROCYTE MEMBRANE NA+,K+-ATPASE AND MG2+-ATPASE AS TARGETS OF ORGANIC SOLVENT IMPACT
【24h】

ASTROCYTE MEMBRANE NA+,K+-ATPASE AND MG2+-ATPASE AS TARGETS OF ORGANIC SOLVENT IMPACT

机译：星形胶质细胞膜NA +，K + -ATPase和MG2 + -ATPase作为有机溶剂作用的靶标

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Astrocytes serve to maintain the proper homeostatic environment for neurons. In these regulations, the astrocytic membrane ATPase plays an important role. In the present study, the astrocyte membrane ATPase as a target of organic solvent impact was investigated. The astrocytes were cultured in the modified Minimum Essential Medium (MEM), and the isolated cell membranes were exposed to solvents at different concentrations (3, 6 and 9 mM) for one hour. The activities of the membrane-bound Na+, K+-ATPase and Mg2+-ATPase were determined after exposure to aromatic, alicyclic and aliphatic hydrocarbons. Aromatic hydrocarbons decreased the enzyme activities dose-dependently according to their lipid solubilities. The slight enzyme-inhibiting effect of aliphatic and alicyclic hydrocarbons was not dependent on the lipophilicity of these solvents. Their molecular structure may have determined the solvent impact. In the present study, the activity of ouabain sensitive Na+, K+-ATPase in astrocytes was only 20-15% of the total ATPase activity, and in neurons 45-55%. The enzyme inhibition caused by organic solvents may disturb the homeostatic regulatory functions of astrocytes and thus have a toxic effect on the CNS. [References: 21]
机译：星形胶质细胞可为神经元维持适当的稳态环境。在这些法规中，星形细胞膜ATPase发挥着重要作用。在本研究中，星形胶质细胞膜ATPase作为有机溶剂影响的目标进行了调查。在改良的最低必需培养基（MEM）中培养星形胶质细胞，并将分离的细胞膜暴露于不同浓度（3、6和9 mM）的溶剂中一小时。暴露于芳族，脂环族和脂族烃后，测定膜结合的Na +，K + -ATPase和Mg2 + -ATPase的活性。芳香烃根据它们的脂质溶解度而剂量依赖性地降低了酶的活性。脂族和脂环族烃的轻微酶抑制作用不取决于这些溶剂的亲脂性。它们的分子结构可能决定了溶剂的影响。在本研究中，星形胶质细胞中哇巴因敏感的Na +，K + -ATPase的活性仅为总ATPase活性的20-15％，而在神经元中的活性为45-55％。由有机溶剂引起的酶抑制作用可能会干扰星形胶质细胞的稳态调节功能，因此对中枢神经系统有毒性作用。 [参考：21]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号