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首页> 外文期刊>Cell biology international. >The strong correlation between alkaline phosphatase activity and cell viability.
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The strong correlation between alkaline phosphatase activity and cell viability.

机译：碱性磷酸酶活性和细胞活力之间的强相关性。

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摘要

We read with interest the recent article (Akcakaya et al., 2007) reporting a quantitative colorimetric method for the measurement of alkaline phosphatase (ALP) activity in eukaryotic cell membranes, especially their results on the relationship between ALP activity of 1G2 hybridomas with respect to the culture conditions. As the serum concentration in the culture media decreased, the authors found that viability of the cells decreased while the specific activity of ALP (measured in OD 405 nm/100 cells) increased. Since a low level or lack of serum leads to nutritional stress, reduced cell viability and apoptosis, the authors suggest that an increase in the ALP activity could be used as an indicator of apoptosis. We show below that this relationship can be described by a simple mathematical function.
机译：我们感兴趣地阅读了最近的文章（Akcakaya等人，2007年），该文章报道了一种定量比色法，用于测量真核细胞膜中的碱性磷酸酶（ALP）活性，尤其是有关1G2杂交瘤相对于1G2杂交瘤的ALP活性之间的关系的结果文化条件。随着培养基中血清浓度的降低，作者发现细胞的活力降低，而ALP的比活性（在OD 405 nm / 100细胞中测得）增加。由于血清水平低或缺乏会导致营养压力，细胞活力降低和细胞凋亡，因此作者建议ALP活性的增加可用作细胞凋亡的指标。下面我们显示可以通过一个简单的数学函数来描述这种关系。

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