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首页> 外文期刊>Cell biology international. >Effects of SIRT6 silencing on collagen metabolism in human dermal fibroblasts.
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Effects of SIRT6 silencing on collagen metabolism in human dermal fibroblasts.

机译：SIRT6沉默对人皮肤成纤维细胞胶原代谢的影响。

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摘要

SIRT6 (sirtuin 6) has been identified as an anti-aging protein, and SIRT6-deficient mice display numerous progeroid and aging-like phenotypes. However, there are no reports correlating skin aging with SIRT6. To explore how SIRT6 affects collagen metabolism and determine its role in skin aging, the levels of COL1A1 (type I collagen), COL3A1 (type III collagen) and TGFbeta1 (transforming growth factor beta1) mRNA expression were detected by RT-Q-PCR (real-time quantitative PCR), and MMP1 (matrix metalloproteinase 1) concentration in HDF (human dermal fibroblast) supernatants was determined by ELISA after blocking SIRT6 using siRNA (small interefering RNA). Compared with the control group, blocking SIRT6 significantly decreased the HC (hydroxyproline content). SIRT6 knockdown in HDFs inhibited the transcription of COL1A1 and prompted MMP1 secretion, but had no effect on COL3A1 and TGFbeta1. Furthermore, NF-kappaB p65 (nuclear factor kappaB p65) protein in the nucleus was increased after blocking SIRT6. The results suggest that SIRT6 knockdown in HDFs influence the synthesis and degradation of collagen by hyperactive NF-kappaB signalling, which leads to a decrease in dermal collagen fibrils. SIRT6 may therefore play an important role in the process of skin anti-aging.
机译：SIRT6（sirtuin 6）已被鉴定为一种抗衰老蛋白，而SIRT6缺陷型小鼠则表现出许多早衰型和衰老样表型。但是，没有报道将皮肤老化与SIRT6相关联。为了探讨SIRT6如何影响胶原蛋白代谢并确定其在皮肤衰老中的作用，通过RT-Q-PCR检测了COL1A1（I型胶原蛋白），COL3A1（III型胶原蛋白）和TGFbeta1（转化生长因子β1）mRNA的表达水平（实时定量PCR）和HDF（人类皮肤成纤维细胞）上清液中的MMP1（基质金属蛋白酶1）浓度是使用siRNA（小分子RNA）阻断SIRT6后通过ELISA测定的。与对照组相比，阻断SIRT6可以显着降低HC（羟脯氨酸含量）。 HDF中的SIRT6抑制可抑制COL1A1的转录并提示MMP1的分泌，但对COL3A1和TGFbeta1没有影响。此外，阻断SIRT6后，细胞核中的NF-κBp65（核因子κBp65）蛋白增加。结果表明，HDF中的SIRT6敲低会通过过度活跃的NF-κB信号传导影响胶原蛋白的合成和降解，从而导致真皮胶原蛋白原纤维减少。因此，SIRT6在皮肤抗衰老过程中可能起重要作用。

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