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Co-expression of nAChRs and molecules of the bitter taste transduction pathway by epithelial cells of intrapulmonary airways

机译：肺内气道上皮细胞与nAChRs和苦味传递途径分子的共表达

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Aims: The ability to sense the bitter taste of nicotine is an important component of addiction to, and withdrawal from, cigarette smoking. alpha-Gustducin and phospholipase C-beta2 (PLC-beta2), molecules involved in the taste transduction pathway, have been identified in airway epithelial solitary chemosensory cells (SCCs). Airway epithelial cells also express multiple nicotinic acetylcholine receptors (nAChRs). However, the relationship between nAChRs and molecules of taste transduction in response to nicotine is not known. This study was designed to determine whether nAChRs and the taste transduction molecules alpha-gustducin, PLC-beta2 and bitter taste receptors (T2R38) reside at sites of the intrapulmonary airways where interaction with the nicotine components of cigarette smoke is likely.Main methods: We used the reverse transcription-polymerase chain reaction (RT-PCR) to detect a-gustducin, PLC-beta2 and T2R38 mRNA and immunohistochemistry to localize expression of these proteins by nAChR expressing cells of the airway.Key findings: RT-PCR demonstrated the presence of mRNA for alpha-gustducin, PLC-beta2 and T2R38. Immunohistochemistry showed the expression of a-gustducin, PLC-beta2 and T2R38 by subsets of epithelial cells at all levels of the intrapulmonary airways including bronchi, terminal and respiratory bronchioles. Double labeling demonstrated the co-expression of alpha-gustducin with alpha3, alpha4, alpha5, alpha7 and beta2, as well as, PLC-beta2 and T2R38 with alpha4, alpha5 and beta2 nAChR subunits.Significance: These findings provide morphological evidence for the presence of molecules of the bitter taste transduction pathway in nAChR expressing SCCs of the intrapulmonary airways. These SCCs may, thus, constitute a peripheral component of the bitter taste signal transduction pathway for nicotine.
机译：目的：感知尼古丁苦味的能力是成瘾和戒烟的重要组成部分。已经在气道上皮孤立化学感应细胞（SCC）中鉴定了与味觉传导途径有关的分子α-古斯汀和磷脂酶C-beta2（PLC-beta2）。气道上皮细胞还表达多种烟碱乙酰胆碱受体（nAChRs）。但是，nAChRs与响应尼古丁的味觉转导分子之间的关系尚不清楚。这项研究旨在确定nAChRs和味觉转导分子α-gustducin，PLC-beta2和苦味受体（T2R38）是否位于可能与香烟烟雾中尼古丁成分相互作用的肺内气道部位。使用逆转录聚合酶链反应（RT-PCR）检测a-gustducin，PLC-beta2和T2R38 mRNA并进行免疫组化定位，以通过气道nAChR表达细胞定位这些蛋白的表达。关键发现：RT-PCR证明了存在α-gustducin，PLC-beta2和T2R38的mRNA表达。免疫组织化学显示，在支气管，末端和呼吸细支气管的所有水平的肺内气道上皮细胞亚群中，a-gustducin，PLC-beta2和T2R38的表达。双重标记证明了α-gustducin与alpha3，alpha4，alpha5，alpha7和beta2以及PLC-beta2和T2R38与alpha4，alpha5和beta2 nAChR亚基的共表达。表达nAChR的肺内气道SCC中苦味传递途径分子的变化。这些SCC因此可以构成尼古丁的苦味信号转导途径的外围成分。

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