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Studies on in vitro effect of free fatty acids on water content and osmotic fragility of rabbit (Lepus cuniculus) erythrocytes.

机译:游离脂肪酸对家兔红细胞水分和渗透性脆性的体外作用研究。

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摘要

The effects of free fatty acids in the blood on osmotic fragility and water content of erythrocytes when combined with hyperthermia were investigated. The isotonic buffer, containing the fatty acid, was added to the erythrocyte suspension to a final concentration of 200 microM/L. The samples were kept for one hour in an incubator at 37 degrees C or 42 degrees C. The osmotic fragility of erythrocytes was determined by coil planet centrifuge system and intracellular water content was measured by gas-liquid chromatography. A high concentration of unsaturated fatty acid and hyperthermia caused the increase in intracellular water and the decrease in osmotic resistance of the red blood cells compared to saturated fatty acid and the control. The present experiment demonstrated that unsaturated fatty acid was one of the principal chemical and metabolic factors which caused sports anemia.
机译:结合热疗,研究了血液中游离脂肪酸对红细胞渗透性脆性和含水量的影响。将包含脂肪酸的等渗缓冲液添加到红细胞悬液中,使其最终浓度为200 microM / L。将样品在37℃或42℃的培养箱中保持1小时。通过螺旋行星式离心机系统测定红细胞的渗透脆性,并通过气液色谱法测定细胞内水含量。与饱和脂肪酸和对照组相比,高浓度的不饱和脂肪酸和热疗引起细胞内水的增加和红细胞渗透阻力的降低。本实验表明,不饱和脂肪酸是引起运动性贫血的主要化学和代谢因素之一。

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