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首页> 外文期刊>Cell and Tissue Research >Comparative analysis of ABCG2-expressing and label-retaining cells in mouse submandibular gland.
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Comparative analysis of ABCG2-expressing and label-retaining cells in mouse submandibular gland.

机译：小鼠下颌下腺中表达ABCG2和保留标签的细胞的比较分析。

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摘要

The submandibular gland (SMG) is a tissue that can be regenerated in a tissue injury model and that has adult stem cells capable of self-renewal and differentiation into functional cells. We have analyzed the localization of label-retaining cells (LRCs), which are putative progenitor cells, by using the BrdU-labeling method. 5-Bromo-2'-deoxyuridine (BrdU) injection followed by a long chasing period permitted the identification of LRCs based on the slow-cycling characteristic. In order to confirm the accurate localization of LRCs, BrdU and SMG-specific markers, including aquaporin5, cytokeratin, and smooth muscle actin, were examined by double-immunofluoresence staining. We found that LRCs were distributed in the acinus, duct, myoepithelium, and connective tissue. Moreover, ABCG2 (a known stem cell marker) was used for the characterization of LRCs and the localization of cells as putative stem/progenitor cells. ABCG2-expressing cells were distributed in various regions of the SMG but did not co-localizewith LRCs. We suggest that putative progenitor cells exist in various regions of the SMG and have diverse capacities to differentiate into specific cells.
机译：颌下腺（SMG）是可以在组织损伤模型中再生的组织，并且具有能够自我更新并分化为功能性细胞的成年干细胞。我们已经通过使用BrdU标记方法分析了标记保留细胞（LRCs）的定位，该标记保留细胞是推定的祖细胞。进行5-Bromo-2'-脱氧尿苷（BrdU）注射，然后进行长时间的追踪，从而可以根据慢循环特性鉴定LRC。为了确认LRC的准确定位，通过双重免疫荧光染色检查了BrdU和SMG特异性标记，包括水通道蛋白5，细胞角蛋白和平滑肌肌动蛋白。我们发现LRCs分布在腺体，导管，肌上皮和结缔组织中。此外，ABCG2（一种已知的干细胞标记）用于表征LRCs和将细胞定位为推定的干/祖细胞。表达ABCG2的细胞分布在SMG的各个区域，但未与LRC共同定位。我们建议推定的祖细胞存在于SMG的各个区域，并具有分化为特定细胞的多种能力。

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