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首页> 外文期刊>Life sciences >Cellular localization of cannabinoid receptors and activated G-proteins in rat anterior cingulate cortex.
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Cellular localization of cannabinoid receptors and activated G-proteins in rat anterior cingulate cortex.

机译：大麻素受体和活化的G蛋白在大鼠前扣带回皮质中的细胞定位。

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摘要

Cannabinoid receptors are found in moderate density throughout the cerebral cortex. The anterior cingulate cortex (ACC) is of particular interest due its high level of cannabinoid receptors and role in behaviors known to be modulated by cannabinoids. These studies were conducted to determine the cellular localization of cannabinoid receptors and to compare the level of cannabinoid receptor binding with receptor-mediated G-protein activity in the rat ACC. Either ibotenic acid or undercut lesions were made in ACC, and brains were processed for [3H]WIN 55,212-2 and WIN 55,212-2-stimulated [35S]GTPgammaS autoradiography. Both cannabinoid receptors and receptor-activated G-proteins were highest in laminae I and VI of ACC in control tissue. Although similar levels of receptor binding were found in these laminae, significantly higher levels of receptor-activated G-proteins were found in lamina VI. Ibotenic acid lesions that destroyed ACC neurons decreased [3H]WIN 55,212-2 binding by 60-70% and eliminated WIN 55,212-2-stimulated [35S]GTPgammaS binding. In contrast, deafferentation of the ACC with undercut lesions had no significant effect on cannabinoid receptor binding or G-protein activation. These results indicate that cannabinoid receptors in laminae I and VI of the ACC are located on somatodendritic elements or axons intrinsic to the ACC. In addition, differences in the relative levels of cannabinoid binding sites and activated G-proteins between cortical laminae indicate that the efficiency of cannabinoid receptors for G-protein activation may vary within a specific brain region.
机译：在整个大脑皮层中发现大麻素受体的密度中等。前扣带回皮层（ACC）由于其高水平的大麻素受体和在已知受大麻素调节的行为中的作用而受到特别关注。进行了这些研究以确定大麻素受体的细胞定位，并比较大麻素受体与大鼠ACC中受体介导的G蛋白活性的结合水平。在ACC中制作了依波酸或咬边病灶，并对大脑进行了[3H] WIN 55,212-2和WIN 55,212-2刺激的[35S] GTPgammaS放射自显影的处理。大麻素受体和受体激活的G蛋白在对照组的ACC层I和VI中最高。尽管在这些薄片中发现了相似水平的受体结合，但是在薄片VI中发现了明显更高水平的受体激活的G蛋白。破坏了ACC神经元的艾波酸损害使[3H] WIN 55,212-2的结合降低了60-70％，并消除了WIN 55,212-2刺激的[35S] GTPgammaS结合。相比之下，带有根切病灶的ACC脱除咖啡因对大麻素受体结合或G蛋白活化没有明显影响。这些结果表明，ACC的层I和VI中的大麻素受体位于ACC固有的树突状元素或轴突上。此外，皮质层之间的大麻素结合位点和活化的G蛋白相对水平的差异表明，大麻素受体G蛋白活化的效率可能在特定的大脑区域内变化。

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