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首页> 外文期刊>Life sciences >Three different pathways for human LDL oxidation are inhibited in vitro by water extracts of the medicinal herb Achyrocline satureoides.
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Three different pathways for human LDL oxidation are inhibited in vitro by water extracts of the medicinal herb Achyrocline satureoides.

机译：药用草药Achyrocline satureoides的水提取物在体外抑制了三种不同的LDL氧化途径。

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In this study we investigated the antioxidant properties of one herbal preparation widely used in complementary and alternative medicine in large areas of the world: Achyrocline satureoides (AS), popularly known as marcela this plant do not include atherosclerosis prevention. Furthermore, no study had been conducted so far exploring the antioxidant activity of Achyrocline satureoides vis-a-vis human LDL oxidation, which is the compelling issue in pinpointing potential cardioprotective new uses for a traditional remedy. We explored the effects of AS extracts on human LDL oxidation, employing 3 different systems which are thought to play a role in oxidation of LDL in the arterial wall: copper, peroxynitrite, and lipoxygenase. Oxidation was monitored by conjugate dienes, TBARS formation and aggregation of apoB using SDS-PAGE. In copper-initiated oxidation a dose dependent inhibition of the initiation and propagation of lipid oxidation is shown by an increase in the lag phase for conjugate diene production which was 60 +/- 15 min in the absence and 120 +/- 20 min in the presence of 4 microg/ml AS extracts (p < 0.001). TBARS production was reduced by 95% after 3 h incubation at 5 microg/ml. Aggregation of apoB was abolished at the same concentrations. SIN-1 (3-morpholinosydnonimine) produces peroxynitrite via generation of NO and O2-. When LDL was incubated in its presence, a milder oxidation was observed as compared with Cu2+, and AS produced over 70% inhibition. Finally, we show a striking dose-dependent inhibitory effect of lipoxygenase conjugate diene production, which is over 95% at AS concentrations of 5 microg/ml. When compared with other antioxidants, AS effect is greater but in the same order of magnitude than that of ascorbic acid and similar to the popular herbal tea Ilex paraguariensis. In all three systems employed an effect is already substantiated at a concentration of the AS extract of 4 microg/ml, which corresponds to a 1/100 dilution of the preparations usually drunk.
机译：在这项研究中，我们调查了在世界大部分地区广泛用于补充和替代药物的一种草药制剂的抗氧化特性：Achyrocline satureoides（AS），俗称Marcela，该植物不包括预防动脉粥样硬化。此外，到目前为止，还没有进行任何研究来研究速乳木果对人的LDL氧化的抗氧化活性，这是在确定传统疗法潜在的心脏保护新用途方面令人信服的问题。我们探索了AS提取物对人LDL氧化的影响，采用了3种不同的系统，它们被认为在动脉壁LDL的氧化中起作用：铜，过氧亚硝酸盐和脂氧合酶。使用SDS-PAGE通过共轭二烯，TBARS的形成和apoB的聚集来监测氧化。在铜引发的氧化中，脂质共轭二烯生产的滞后阶段增加显示了脂质氧化的发生和扩散的剂量依赖性抑制，共轭二烯生产的滞后阶段增加了60 +/- 15分钟，而在无氧条件下为120 +/- 20分钟。存在4 microg / ml AS提取物（p <0.001）。以5 microg / ml孵育3小时后，TBARS产量降低了95％。在相同浓度下消除了载脂蛋白B的聚集。 SIN-1（3-吗啉代亚砜亚胺）通过生成NO和O2-生成过氧亚硝酸盐。当LDL在其存在下孵育时，与Cu2 +相比，观察到更温和的氧化，并且AS产生超过70％的抑制。最后，我们显示出惊人的剂量依赖性的脂氧合酶共轭二烯生产抑制作用，在AS浓度为5微克/毫升时超过95％。与其他抗氧化剂相比，AS效果要好于抗坏血酸，但数量级相同，与流行的凉茶Ipara paraguariensis相似。在所有三种使用的系统中，AS提取物的浓度为4微克/毫升时，已经证实了效果，这相当于通常喝的1/100稀释制剂。

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