...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell and Tissue Research >Vitrified sheep isolated secondary follicles are able to grow and form antrum after a short period of in vitro culture
【24h】

Vitrified sheep isolated secondary follicles are able to grow and form antrum after a short period of in vitro culture

机译：在短时间的体外培养后，玻璃化的绵羊分离出的次级卵泡能够生长并形成胃窦

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The risk of reintroducing malignant cells after ovarian graft into patients following post-cancer treatment is an obstacle for clinical applications (autotransplantation). In this context, in vitro follicle culture would be an alternative to transplantation in order to minimize such risks. Therefore, the aim of this study was to compare the development of secondary follicles after vitrification in isolated form (without stroma) with vitrification in in situ form (within fragments of ovarian tissue). Follicles were first isolated from ovarian fragments from mixed-breed ewes and then vitrified; these comprised the Follicle-Vitrification group (Follicle-Vit), or fragments of ovarian tissue were first vitrified, followed by isolation of the follicles, resulting in the Tissue-Vitrification group (Tissue-Vit). Control and vitrified groups were submitted to in vitro culture (6 days) and follicular morphology, viability, antrum formation, follicle and oocyte diameter, growth rate, ultrastructural characteristics and cell proliferation were evaluated. The percentages of morphologically normal follicles and antrum formation were similar among groups. Follicular viability and oocyte diameter were similar between Follicle-Vit and Tissue-Vit. The follicular diameter and growth rate of Follicle-Vit were similar to the Control, while those of Tissue-Vit were significantly lower compared to the Control. Both vitrified groups had an augmented rate of granulosa cellular proliferation compared to Control. Secondary follicles can be successfully vitrified before or after isolation from the ovarian tissue without impairing their ability to survive and grow during in vitro culture.
机译：癌后治疗后，将卵巢移植后的恶性细胞重新引入患者体内的风险是临床应用（自体移植）的障碍。在这种情况下，体外卵泡培养可以替代移植，以最大程度地降低此类风险。因此，本研究的目的是比较玻璃化分离形式（无基质）和原位玻璃化（卵巢组织内）后继发卵泡的发育。首先从混合母羊的卵巢碎片中分离出卵泡，然后进行玻璃化。这些包括卵泡玻璃化组（Follicle-Vit），或首先将卵巢组织碎片玻璃化，然后分离卵泡，从而形成组织玻璃化组（Tissue-Vit）。对照组和玻璃化组接受体外培养（6天），并评估卵泡形态，生存力，胃窦形成，卵泡和卵母细胞直径，生长速率，超微结构特征和细胞增殖。各组之间形态正常的卵泡和胃窦形成的百分比相似。 Follicle-Vit和Tissue-Vit之间的卵泡活力和卵母细胞直径相似。与对照相比，Follicle-Vit的卵泡直径和生长速率与对照相似，而组织-Vit的卵泡直径和生长速率则明显低于对照。与对照组相比，两个玻璃化组的颗粒细胞增殖率均增加。从卵巢组织分离之前或之后，次要卵泡可以成功地玻璃化，而不会损害它们在体外培养过程中存活和生长的能力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号