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Human ovary and placenta express messenger RNA for multiple activin receptors.

机译:人卵巢和胎盘表达多种激活素受体的信使RNA。

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In the present study, we examined the expression of activin receptor (ActR) mRNAs in human ovary and placenta. Primers specific for two type I and two type II activin receptors (ActR-I, ActR-IB, ActR-II, and ActR-IIB) were used in polymerase chain reaction (PCR) to amplify cDNAs prepared from granulosa-luteal cells, placental tissues and isolated trophoblast cells. PCR products with the expected sizes for ActR-I, ActR-IB, ActR-II, and ActR-IIB mRNAs were detected in freshly dissociated and 5-day cultured granulosa-luteal cells; and in trophoblast cells from both first trimester and term placentas. The identity of these PCR products were confirmed by Southern blot hybridization, as well as cloning and sequencing. These results suggest that multiple activin receptors are present in human ovary and placenta and may mediate activin function in these tissues. The demonstration of activin receptor mRNAs in granulosa-luteal and trophoblast cells further supports the notion that activin is an important local regulator in the human ovary and placenta.
机译:在本研究中,我们检查了激活素受体(ActR)mRNA在人卵巢和胎盘中的表达。对两种I型和II型激活素受体(ActR-1,ActR-IB,ActR-II和ActR-IIB)具有特异性的引物用于聚合酶链反应(PCR)中,以扩增由颗粒-黄体细胞,胎盘制备的cDNA。组织和分离的滋养细胞。在新鲜分离的和培养5天的颗粒-黄体细胞中检测到具有ActR-I,ActR-IB,ActR-II和ActR-IIB mRNA预期大小的PCR产物。以及早孕期和胎盘期的滋养层细胞。这些PCR产物的身份通过Southern印迹杂交以及克隆和测序证实。这些结果表明人卵巢和胎盘中存在多种激活素受体,并可能在这些组织中介导激活素功能。在颗粒-黄体和滋养细胞中激活素受体mRNA的证实进一步证实了激活素是人卵巢和胎盘中重要的局部调节剂的观点。

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