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首页> 外文期刊>Life sciences >Effect of naturally occurring organosulfur compounds on nitric oxide production in lipopolysaccharide-activated macrophages.
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Effect of naturally occurring organosulfur compounds on nitric oxide production in lipopolysaccharide-activated macrophages.

机译：天然存在的有机硫化合物对脂多糖激活的巨噬细胞中一氧化氮生成的影响。

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摘要

Excessive nitric oxide (NO) production is involved in cellular injury and possibly in the multistage process of carcinogenesis. In this study, we investigated the effect of organosulfur compounds (S-allyl cysteine, allyl sulfide, diallyl disulfide, allyl isothiocyanate, phenyl isothiocyanate, and benzyl isothiocyanate) that are found in allium or cruciferous vegetables on NO production in J774.1 macrophages activated with lipopolysaccharide (LPS). Diallyl disulfide, allyl, phenyl, and benzyl isothiocyanates inhibited NO production, as evaluated by nitrite formation at 25 &mgr;M. Allyl and benzyl isothiocyanates, the most active of the six organosulfur compounds, exhibited dose-dependent inhibition and had IC(50) values of 1.6 and 2.7 &mgr;M, respectively. Western blot analysis suggested that suppression of the induction of inducible NO synthase (iNOS) expression is responsible for the inhibition of NO production by allyl and benzyl isothiocyanates. In contrast, these isothiocyanates increased LPS-stimulated tumor necrosis factor alpha (TNF-alpha) release, suggesting their selective action on genes activated by LPS. Our results demonstrate that certain organosulfur compounds inhibit NO synthesis in LPS-activated macrophages, and the inhibitory effect may be a significant component of their anticarcinogenic activity.
机译：一氧化氮（NO）的过量产生与细胞损伤有关，并可能与致癌作用的多阶段过程有关。在这项研究中，我们调查了葱属或十字花科蔬菜中发现的有机硫化合物（S-烯丙基半胱氨酸，烯丙基硫醚，二烯丙基二硫醚，烯丙基异硫氰酸酯，苯基异硫氰酸酯和苄基异硫氰酸酯）对活化的J774.1巨噬细胞中NO产生的影响。与脂多糖（LPS）。二烯丙基二硫化物，烯丙基，苯基和异硫氰酸苄酯抑制NO的产生，如在25 M下亚硝酸盐的形成所评估的。六个有机硫化合物中活性最高的烯丙基和异硫氰酸苄酯显示出剂量依赖性抑制作用，IC（50）值分别为1.6和2.7M。蛋白质印迹分析表明，抑制诱导型一氧化氮合酶（iNOS）表达的诱导是烯丙基和异硫氰酸烯丙酯对一氧化氮产生的抑制作用。相反，这些异硫氰酸盐增加了LPS刺激的肿瘤坏死因子α（TNF-alpha）的释放，表明它们对LPS激活的基因具有选择性。我们的结果表明，某些有机硫化合物会抑制LPS活化的巨噬细胞中的NO合成，而抑制作用可能是其抗癌活性的重要组成部分。

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