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首页> 外文期刊>Life sciences >Possible use of quercetin, an antioxidant, for protection of cells suffering from overload of intracellular Ca2+: a model experiment.
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Possible use of quercetin, an antioxidant, for protection of cells suffering from overload of intracellular Ca2+: a model experiment.

机译：槲皮素（一种抗氧化剂）可能用于保护遭受细胞内Ca2 +超负荷的细胞：一个模型实验。

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Quercetin is known to protect the cells suffering from oxidative stress. The oxidative stress elevates intracellular Ca(2+) concentration, one of the phenomena responsible for cell death. Therefore, we hypothesized that quercetin would protect the cells suffering from overload of intracellular Ca(2+). To test the hypothesis, the effects of quercetin on the cells suffering from oxidative stress and intracellular Ca(2+) overload were examined by using a flow cytometer with appropriate fluorescence probes (propidium iodide, fluo-3-AM, and annexin V-FITC) and rat thymocytes. The concentrations (1-30 microM) of quercetin to protect the cells suffering from intracellular Ca(2+) overload by A23187, a calcium ionophore, were similar to those for the cells suffering from oxidative stress by H(2)O(2). The cell death respectively induced by H(2)O(2) and A23187 was significantly suppressed by removal of external Ca(2+). Furthermore, quercetin greatly delayed the process of Ca(2+)-dependent cell death although it did not significantly affect the elevation of intracellular Ca(2+) concentration by H(2)O(2) and A23187, respectively. It is concluded that quercetin can protect the cells from oxidative injury in spite of increased concentration of intracellular Ca(2+). Results suggest that quercetin is also used for protection of cells suffering from overload of intracellular Ca(2+).
机译：已知槲皮素可保护遭受氧化应激的细胞。氧化应激会升高细胞内Ca（2+）的浓度，这是造成细胞死亡的现象之一。因此，我们假设槲皮素将保护遭受细胞内Ca（2+）超负荷的细胞。为了验证该假设，使用流式细胞仪和适当的荧光探针（碘化丙啶，fluo-3-AM和膜联蛋白V-FITC）检查了槲皮素对遭受氧化应激和细胞内Ca（2+）超负荷的细胞的影响）和大鼠胸腺细胞。槲皮素的浓度（1-30 microM）通过细胞钙离子载体A23187保护遭受细胞内Ca（2+）超负荷的细胞，与遭受H（2）O（2）氧化应激的细胞的浓度相似。分别由H（2）O（2）和A23187诱导的细胞死亡通过去除外部Ca（2+）得到显着抑制。此外，槲皮素极大地延迟了Ca（2+）依赖性细胞死亡的过程，尽管它并没有显着影响H（2）O（2）和A23187分别升高细胞内Ca（2+）的浓度。结论是槲皮素可以保护细胞免受氧化损伤，尽管细胞内Ca（2+）的浓度增加。结果表明槲皮素还用于保护细胞内Ca（2+）超负荷的细胞。

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