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首页> 外文期刊>Life sciences >Effect of recombinant human SDF-1a on re-endothelialization after sirolimus-eluting stent implantation in rabbit aorta abdominalis.
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Effect of recombinant human SDF-1a on re-endothelialization after sirolimus-eluting stent implantation in rabbit aorta abdominalis.

机译：重组人SDF-1a对西罗莫司洗脱支架植入兔腹主动脉后再内皮化的影响。

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AIMS: Although the use of drug-eluting stents (DES) has been shown to limit neointimal hyperplasia in clinical coronary artery disease treatment, currently available DES may adversely affect re-endothelialization (RE) and thus increase fateful stent thrombosis. As stromal cell derived factor 1a (SDF-1a) plays an essential role in the regulation of endothelial progenitor cells (EPCs) mobilization, homing, and differentiation in response to vascular injury, we assumed that SDF-1a may enhance EPCs adhesion and attenuate delayed RE associated with DES. MAIN METHODS: Biologically active recombinant human SDF-1a (rhSDF-1a) was first produced using an Escherichia coli expression system. Twenty-four male rabbits were then underwent sirolimus-eluting stents implantation in aorta abdominalis. After operation, they were randomly divided into two groups and subcutaneously injected daily with 50 mug/kg rhSDF-1a or the same volume of saline for 7 days. KEY FINDINGS: With the application of scanning electron microscopy (SEM) and histological analysis, we found that rhSDF-1a significantly promoted RE on days 7, 14, 28 and inhibited neointimal hyperplasia on day 28 after stent implantation. SIGNIFICANCE: Our results revealed a potential role of rhSDF-1a in facilitating RE and inhibiting neointimal proliferation after DES implantation, leading to a conclusion that this protein may be a potential candidate agent for the treatment of in-stent restenosis and stent thrombosis.
机译：目的：尽管已证明在临床冠状动脉疾病治疗中使用药物洗脱支架（DES）可以限制新内膜增生，但目前可用的DES可能会对再内皮化（RE）产生不利影响，从而增加命运重大的支架血栓形成。由于基质细胞衍生因子1a（SDF-1a）在调节血管内皮祖细胞（EPC）的动员，归巢和分化以应对血管损伤中起着至关重要的作用，因此我们假设SDF-1a可能会增强EPC的黏附并减轻延迟RE与DES关联。主要方法：首先使用大肠杆菌表达系统生产具有生物活性的重组人SDF-1a（rhSDF-1a）。然后将24只雄性兔子在腹主动脉中接受西罗莫司洗脱支架植入。手术后，将它们随机分为两组，每天皮下注射50杯/千克rhSDF-1a或等体积的生理盐水，持续7天。主要发现：通过扫描电子显微镜（SEM）和组织学分析，我们发现rhSDF-1a在支架植入后第7、14、28天显着促进RE，并在第28天抑制新生内膜增生。意义：我们的结果显示，rhSDF-1a在DES植入后促进RE和抑制新内膜增生方面具有潜在作用，从而得出结论，该蛋白可能是治疗支架内再狭窄和支架血栓的潜在候选药物。

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